Highly Efficient Tumor-Targeted Nanoparticles Deliver TRAIL as Gene Therapy For Hepatocellular Carcinoma

• Main Authors: Liu, Chun Hung, 劉俊宏
• Other Authors: Yunching Chen
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/62327978846859283295

碩士 === 國立清華大學 === 生物醫學工程研究所 === 104 === Gene therapy for cancer treatment has become the very promising approach of hepatocellular carcinoma (HCC). However, the therapeutic plasmid DNA delivery into cancer cell and regulate the expression is a challenge of many studies. We utilize SP94 peptide as targeted ligand modifies liposome calcium phosphate nanoparticles (LCP-NPs) to specifically target HCC. We also use protamine to improve transfection efficiency which contains several nuclear localization signal (NLS). Moreover, we want to deliver an apoptosis inducer gene -TRAIL (Tumor necrosis factor-related apoptosis-inducing ligand) to induce tumor cell apoptosis. These results demonstrated that targeting LCP-NPs can specifically delivery therapeutic plasmid DNA into HCC to regulate cancer cell expression.