Summary: | 碩士 === 國立臺南大學 === 生物科技學系碩士班 === 104 === Plasticizes (PAEs) bear numeral functions, such as adjusting the hardness and luster of product, cost reduction...etc, allowing them to be widely used in industries, for instances, plastic additives. However, this prevailing substance has great impact on our environments. Absorption by plants and animals can lead to tissue lesions after accumulation within individuals. Diethyl phthalate (DEP), a member of PAEs, is commonly used in solvents, nursing supplies, ink, and other items. Though having lower toxicity compared to other plasticizers, DEP can still pose safety concerns due to discharging into rivers and lakes after over-usage.
In this study, we choose Spirodela polyrhiza L. Schleid. as our sample. By using JIP-test, we analyzed the effect caused by DEP on the donor side, the receptor side and reaction center of photosystem II (PSII) based on fast chlorophyll fluorescence induction dynamics analysis. The result indicates that DEP will affect the electron transfer efficiency of oxygen-evolving complex (OEC) downstream receptors. Reaction center activity was not good during primary light reaction at J site, suggesting that the redox efficiency was decreased between QA and QB. On the other hand, the rise of O site of Spirodela polyrhiza in fluorescent curve under DEP treatment implies that the light absorption complexes of PSII (LHCII) may be damaged, resulting in the rise of basic fluorescent value. Meanwhile, dissipated energy in PSII increased drastically, thus decreasing the energy transferred by electron transport chain. In summary, the photosynthesis efficiency of Spirodela polyrhiza decreases significantly under DEP treatments.
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