| Summary: | 碩士 === 國立臺南大學 === 生態科學與技術學系環境生態碩士在職專班 === 104 === We utilize the Carboxymethyl cellulose sodium(CMC) as the carbon source and investigate the hydrolysis ability and feature of the Cellulomonas uda (Uda) , and the Uda was selected from the local bacterial strain. The amount of reducing sugar would be gradually decreased after transplanting the Uda’s cultured generation. Due to the previous phenomenon, the growth retardation of the Uda of the anaerobic culturing process would be the possible reason for the less production of cellulose hydrolysis enzyme. Based on the facultative aerobic characteristic of the Uda, we need to modify the cultivating method.
Aerobic culturing could increase the growth rate of the Uda and produce the large amount of cellulose hydrolytic enzyme in a short time, and the cumulative amount of the cellulose hydrolytic enzyme would be reached to 692 mg/L. However, the production amount of reducing sugar would be reduced after aerobic cultivating through generations transplanted, it would be probably due to inappropriate proportion of the carbon and the nitrogen sources of the liquid medium. In order to solve the forgoing problem, we added the microorganism to utilize the glucose as the carbon source effectively and peptone and yeast extract as the organic nitrogen source. While utilizing the yeast extract as the organic nitrogen source, it showed no significant increase for the cell concentration OD660 of the Uda and the production amount of reducing sugar. On the other hand, the addition of the peptone could make reducing sugar increase and become stable till 100 hours, and the cumulative yield rate was 6.9 mg / L-hr.
The Uda could not hydrolyze effectively by utilizing the bagasse as the carbon source, and it would be possible to need more time to cultivate the Uda to hydrolyze the bagasse to transform to reducing sugar. However, we need to further evaluate the conversion rate of the reducing sugar for the cultivated Uda. In addition, the hydrogen production capacity test mixed the anaerobic producing hydrogen bacteria with the Uda, and the test result showed that the addition of the Uda could really help anaerobic producing hydrogen bacteria to produce the hydrogen. But the hydrolytic enzyme system of the Uda for the previous experiment was not a complete hydrolytic system and had the poor efficiency of the hydrogen production. Hence, we need to supply the extra Uda to form a complete enzyme hydrolytic system in a synergistic manner.
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