Biosensors in Extended Nanofluidic Device

• Main Authors: Yii-Lih Lin, 林以立
• Other Authors: Chia-fu Chou
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/06348445674057549826

博士 === 國立臺灣大學 === 化學研究所 === 104 === Nano/microfluidic devices have shown their superior capabilities in various applications. As compared to conventional biochemical assays, nano/microfluidic devices pose minimized dimensions, reduce the required sample consumption and shorten the overall assay time. Recent progress in lab-on-a-chip technologies further allows the miniaturization of fluidic channels to nanoscale level where molecular reactions become extremely efficiently as the inter-molecular distance are confined to be comparable to the molecular diffusion distance. This study represents a methodology to implement antibody microarray into extended nanoslits. Through moderate surface modification, fluidic chips are compatible with microarray spotting and the following chip encapsulation procedure. A room temperature chip-bonding process using polysilsesquioxane as gasket layer was integrated in the procedure to preserve the activities of the immobilized biomolecules. Furthermore, the antigen-antibody binding kinetics in the confined space was observed via real-time fluorescence imaging. In addition to antibody specificity preservation, extremely high reaction efficiency was observed as upstream microspots collect most fluorescently labeled target molecules and creates a dark depletion zone in the downstream. The kinetics was simulated through a finite element model and also a simplified one-dimensional convection-reaction model, which confirms that diffusion is efficient in the confined space. This work provides a combined strategy to incorporate protein microarrays within extended nanoslits, and a side-by-side simulation to study the kinetics.