| Summary: | 碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 104 === In order to overcome the shortage of organ donation and injury of patients after partial hepatectomy (PH) treatment, we aimed at developing a liver film for hepato-cytes regeneration through liver tissue engineering. In this study, a HGF/heparin-immobilized decellularized liver matrix film (HGF/heparin complex coated on DLM film) was developed for hepatocytes regeneration in liver injury. In the result, (1) the amounts of immobilized heparin on DLM film was 30 μg/cm2 when the initial heparin concentrations were 1 mg/mL. (2) The relative cell viability and albu-min synthesis of the hepatocytes on HGF/heparin-immobilized DLM film was 20-30 % and 20 % superior than on normal dish at 3 days of culture, respectively. (3) The lactate dehydrogenase activity of the D-galactosamine-induced injury of hepatocytes on hepa-rin-immobilized DLM film cultures was 10 milliunits/mL, which was 50 % lower than that in the D-galactosamine-induced injury of hepatocytes on normal dish cultures, and albumin synthesis can recover to the same level as non-toxic hepatocytes cultured on normal dish. The HGF/heparin-immobilized DLM film showed highly potential in maintaining hepatocyte culture and also in repairing injured hepatocytes from D-galactosamine. It is believed that this HGF/heparin-immobilized DLM film has promising potential for hepatocyte transplantation, and could be applied for future use in liver tissue engineering.
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