| Summary: | 碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 104 === Signal transducer and activator of transcription 3 (STAT3) is an important transcription factor in the cell. Abnormal activation of STAT3 has been proven to be a factor leading to cancer cell and regulate cell proliferation, differentiation, apoptosis, immune and inflammatory responses. Since over-activated STAT3 is often found in malignant tumor, it has the strong potential to be a biomarker. STAT3-decoy oligonucleotide (STAT3-dODN) is a specific sequence which is simulate to promoter of target gene. It has ability binding to STAT3 DNA binding domain, and also inhibits the gene expression.
Aptamers, stable single-stranded nucleotides, which have high binding affinity and specificity to target, are selected through Systematic Evolution of Ligands by Exponential enrichment (SELEX).
In the study, it shows that it may not be suitable for simultaneous screening of six STAT proteins. In addition, STAT proteins may be not suitable for being each other counter selection protein.
In the present study, we demonstrated micro-bead SELEX with different library pool named as N40 and dODN pool. The result showed that we get candidate aptamers in early round of selection (<5). From the ELONA experiment, we get two aptamers named dODN-18 and dODN-20 which have higher affinity to STAT3 protein then dODN-pi. With MTT assay, we also realize the influence of dODN-18 and dODN-20 to cells that it may cause the cell death. Therefore, there are several applications for diagnosis and treatment in the future.
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