Biochemical System Synthesis using Enzyme Reactions

• Main Authors: Yu-Chan Wang, 王瑜禪
• Other Authors: Jie-Hong Jiang
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/92637795891985322230

碩士 === 國立臺灣大學 === 電子工程學研究所 === 104 === The objective of biochemical system synthesis in synthetic biology is to transfer an abstract form of desired system behavior into a designable biochemical reaction system, usually described in CRNs. This work is to map the synthesized CRNs into the enzyme reaction motifs, making it closer to their final enzymatic realizations. In this thesis, we present methods to synthesize three different ways of system specifications into enzyme reaction realizations. The first framework is for reconfigurable logic circuit synthesis. It combines enzyme-synthesized configurable logic units to evaluate desired Boolean formulas. The second one is for level-based neuromorphic computing system synthesis. It realizes neuromorphic algorithms based on binary neurons. The last one is for real valued polynomial synthesis. Complex polynomials are implemented by combining several basic arithmetic operations described in enzyme motifs together. For each framework, we simulate with a simple example and verify the output correctness.