| Summary: | 碩士 === 國立臺灣大學 === 應用力學研究所 === 104 === In this thesis, we have successfully developed a novel micro-fluidic cytometer, which has advantages of rapid, high-throughput and low cost. Cytometer has existed in the industry for more than 70 years, but the current cytometers are expensive, large and low efficiency. To reach high-throughput, we made 32 cascade sample channels to achieve 32 times efficiency. However, it will cause biopsy floating around the channel that makes following detection harder, therefore, we apply 3D fluid focusing to solve this problem. Our design is different from geometry cytometer which can not reach high-throughput; and the inertial cytometer which is an unfriendly-environment to cells. Thus, we design 3D channels filled with environmental solution of biopsy to fluid focus, which means that x-y plane focusing by sheath flow and y-z plane focusing by vertical flow encircling the biopsy, circumscribes the position of biopsy for following detection. In order to enhance N.A. of this system, 32 micro-ball lens was embedded under 32 channels, which can shorten the focus length for compressing the volume of chip. We demonstrate highly focusing by counting fluorescent microsphere which has identical intensity, and demonstrate high throughput by counting labeled cells (Raw). We count the biopsy by exciting it by laser and collecting its fluorescence when it passing through micro-ball lens array. Then analyze the intensity of fluorescence which determines the degree of fluid focusing. In summary, the system could absolutely confines fluorescent microsphere in the focusing area, and it could reach 140 million cell/s by detecting Raw cell of 5x108 cell/ml concentration.
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