The Investigation of Field Effect Electrode for CNT-Based Biosensors

• Main Authors: WANG,SHENG-YU, 王笙宇
• Other Authors: WU,HUE-MIN
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/11956615664618034611

碩士 === 中國文化大學 === 化學工程與材料工程學系奈米材料碩士班 === 104 === Functional single-walled carbon nanotubes (SWNTs) exhibit a unique combination of excellent mechanical, electrical and electrochemical properties, which has stimulated increasing interest in the application of SWNTs as components of biosensors. Considerable advancement has been made toward their synthesis and processing to fabricate molecular devices such as field-effect transistors. This progress has led to the recent emergence of bio/abio hybrid technology that bridges the sciences of CNTs and biology by pairing the advantages of CNTs and biological materials, such as DNA and proteins. However, one of main challenges is imaging in the reaction environment with resolution down to nanometer scale for noninvasive monitoring of the biomolecule. One the hand, although CNTs could be manipulated by using and atomic-force microscope tip, it is still difficult to handle or align individual CNTs to ideal locations. In this research, we report a simple and general approach to π-π stacking functionalization of the sidewalls of SWNTs by N-succinimidyl-1-pyrenebutanoate (PSE), and subsequent immobilization of insulin-like growth factor 1 receptor (IGF1R) onto SWNTs with a high degree of control and specificity. The selection of PSE provides visualization and characterization of individual CNTs based on its strong luminescence. In addition, we designed a simple and efficient electrode with a staggered pattern to increase the effect of electrophoresis by using electric field for the macroscopic alignment of SWNTs to complete a field-effect device for CNT-based biosensors. The results of four-point probe method demonstrated high sensitivity of detection. The functional of SWNTs was investigated by Fourier transform infrared spectroscopy (FTIR)、Raman spectroscopy、UV-visible spectroscopy.