Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics
碩士 === 靜宜大學 === 化粧品科學系 === 104 === This study is to propose a novel method for determination regulations limits used sebum softener, such as Mandelic Acid,Salicylic acid and Resorcinol on detection in cosmetics. For this purpose, a fastly and convenient analytical instrument Capillary Electrophorsis...
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ndltd-TW-104PU0001150092017-09-17T04:24:19Z http://ndltd.ncl.edu.tw/handle/32823948346681845003 Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics 實驗設計輔助分散式液液微萃取結合毛細管電泳儀偵測化妝品中的角質軟化劑 LIN, YU-CHING 林雨靜 碩士 靜宜大學 化粧品科學系 104 This study is to propose a novel method for determination regulations limits used sebum softener, such as Mandelic Acid,Salicylic acid and Resorcinol on detection in cosmetics. For this purpose, a fastly and convenient analytical instrument Capillary Electrophorsis (CE) combine with Dispersive liquid-liquid micro extraction (DLLME) was designed, and this parameters were studied by orthogonal experimental design and statistical analysis to optimize. The Taguchi Method was applied to at L16(215) and L18 (21×37) orthogonal experimental design. According to the number of chromatographic peaks, peak signal strength and retention time, separation voltage at -25 kV and a background electrolyte(BGE, 10 mM phosphate buffer at pH 9) were used. Sebum softeners in cosmetics were analyzed by CE at 220 nm. For measured in quantifying the collected solvent, the present work utilizes a self-designed glass tube in the extraction process. The optimum parameters for MA, SA and Res were investigated. Parameters influencing the extraction efficiency of DLLME-BE-CE, such as the extraction and dispersive solvent, solvent volume, and salt-addition were thoroughly investigated and optimized. After that, the maximum extraction efficiency was obtained by loading 20 µL 10mM HCl 3 mL sample solution, by the addition of 200 μL Octanol (extractant) and 80 μL Acetonitrile. Then the device was sealed with parafilm, and the cloudy solution was centrifuged at 3000 rpm for 3min and the separated extractant was injected directly into CE for analysis. By the ANOVA(analysis of variance),we can find the parameters which influence the extraction solvent type and the optimum combination of parameter values. Under the selected conditions,determination of sebum softeners ranged in 10-2000 ng/mL. The coefficient of determination were obtained above 0.9935. Detection limits were achieved at the level of 0.5-6.7 ng/mL. Quantitative limits were achieved at the level of 2.7-18.4 ng/mL. The relative standard deviations of intra-day and inter-day precisions were 4.58-8.44 % and 7.35-15.68 %. The extraction time was less than 10 minutes. The results demonstrated that the proposed DLLME-BE-CE method provides a simple, rapid, inexpensive, convenient, eco-friendly and less matrix interferences process for the determination sebum softener in cosmetic samples. TSAI, FENG-JIE 蔡鳳婕 2016 學位論文 ; thesis 113 zh-TW |
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碩士 === 靜宜大學 === 化粧品科學系 === 104 === This study is to propose a novel method for determination regulations limits used sebum softener, such as Mandelic Acid,Salicylic acid and Resorcinol on detection in cosmetics. For this purpose, a fastly and convenient analytical instrument Capillary Electrophorsis (CE) combine with Dispersive liquid-liquid micro extraction (DLLME) was designed, and this parameters were studied by orthogonal experimental design and statistical analysis to optimize. The Taguchi Method was applied to at L16(215) and L18 (21×37) orthogonal experimental design.
According to the number of chromatographic peaks, peak signal strength and retention time, separation voltage at -25 kV and a background electrolyte(BGE, 10 mM phosphate buffer at pH 9) were used. Sebum softeners in cosmetics were analyzed by CE at 220 nm. For measured in quantifying the collected solvent, the present work utilizes a self-designed glass tube in the extraction process. The optimum parameters for MA, SA and Res were investigated.
Parameters influencing the extraction efficiency of DLLME-BE-CE, such as the extraction and dispersive solvent, solvent volume, and salt-addition were thoroughly investigated and optimized. After that, the maximum extraction efficiency was obtained by loading 20 µL 10mM HCl 3 mL sample solution, by the addition of 200 μL Octanol (extractant) and 80 μL Acetonitrile. Then the device was sealed with parafilm, and the cloudy solution was centrifuged at 3000 rpm for 3min and the separated extractant was injected directly into CE for analysis.
By the ANOVA(analysis of variance),we can find the parameters which influence the extraction solvent type and the optimum combination of parameter values. Under the selected conditions,determination of sebum softeners ranged in 10-2000 ng/mL. The coefficient of determination were obtained above 0.9935. Detection limits were achieved at the level of 0.5-6.7 ng/mL. Quantitative limits were achieved at the level of 2.7-18.4 ng/mL. The relative standard deviations of intra-day and inter-day precisions were 4.58-8.44 % and 7.35-15.68 %. The extraction time was less than 10 minutes.
The results demonstrated that the proposed DLLME-BE-CE method provides a simple, rapid, inexpensive, convenient, eco-friendly and less matrix interferences process for the determination sebum softener in cosmetic samples.
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author2 |
TSAI, FENG-JIE |
author_facet |
TSAI, FENG-JIE LIN, YU-CHING 林雨靜 |
author |
LIN, YU-CHING 林雨靜 |
spellingShingle |
LIN, YU-CHING 林雨靜 Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics |
author_sort |
LIN, YU-CHING |
title |
Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics |
title_short |
Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics |
title_full |
Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics |
title_fullStr |
Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics |
title_full_unstemmed |
Experimental Design Assisted Dispersive Liquid Liquid Microextraction and Capillary Electrophorsis for Determination of Sebum Softener in Cosmetics |
title_sort |
experimental design assisted dispersive liquid liquid microextraction and capillary electrophorsis for determination of sebum softener in cosmetics |
publishDate |
2016 |
url |
http://ndltd.ncl.edu.tw/handle/32823948346681845003 |
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