| Summary: | 碩士 === 靜宜大學 === 食品營養學系 === 104 === Alzheimer’s disease is the most common neurodegenerative disease. Many studies have indicated that Alzheimer’s disease was related to neurotransmitter acetylcholine content. Recently, there is an increasing interest in the study how to protect nerve cells and improve the neurodegenerative disease. Glechoma hederacea belongs to the Lamiaceae family, is used for medication purpose widely and possesses several health benefits. This study was aimed to evaluate the effects of the crude extract (HWG, GH50, GH95) and partitioned fractional extract (EA, n-butanol, H2O) of G. hederacea on H2O2-induced toxicity and oxidative damage in rat pheochromocytoma line ( PC12 cells). Furthermore, we also identified the phenolic compounds of the G. hederacea extracts by using reverse-phase high performance liquid chromatography (HPLC) coupled with UV/vis detector.
The results demonstrated that three partitioned fractional extracts from G. hederacea possessed good inhibition of acetylcholinesterase, DPPH and ABTS+ radical, and have well reducing power and inhibit lipid peroxidation activities. It was obvious that EA extract was more effective protection ability. In the cell model, the EA extract didn’t possess the cytotoxicity in PC12 cells and can recover the cell morphology and the cell viability of H2O2-treated PC12 cells. In addition, EA extract can improve the glutathione (GSH) levels, decrease the malondialdehyde (MDA) levels and regulate the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase activities. Moreover, the EA extract can prevents H2O2-induced DNA damage and apoptosis in PC12 cells. The rosmarinic acid content in G. hederacea extracts was the highest.
In conclusion, EA extract possessed the AchE inhibitory activity and balanced the oxidant-antioxidant defense in H2O2-treated PC12 cells. EA extract inhibited the cell apoptosis and DNA damage. The EA extract may have the antioxidant activity and neuroprotective potential.
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