| Summary: | 碩士 === 南臺科技大學 === 生物科技系 === 104 === Prostate cancer (CaP) is known to be the most common malignancy in men and the second leading cause of cancer mortalities among U.S. males with a similar trend in many Western countries and in Taiwan. Chemoprevention aims to decrease both incidence and mortality of cancer through the use of pharmacological agents to prevent, reverse, or delay the carcinogenic process. Cordyceps militaris is a popular traditional Chinese medicinal fungus. It has been widely used as an herbal drug and a tonic food in East Asia and also being studied in the West recently because of its various pharmacological activities such as antitumor, antioxidation and immunomodulatory effects. Nowadays there is no literature showing that C. militaris foils prostate cancer, and incidences increase every year in Taiwan. Thus in this study, we intended to explore whether C. militaris fruit body can inhibit prostate cancer.
In this study, cell proliferation and the expressions of various proteins associated with apoptosis and cell cycle regulation were observed in the ethanol extract (CM_EE) of C. militaris fruit body-treated prostate cancer cells, which was grown on pupa of cicada and provided by the center for biotechnology development of Nan Pao Resins Chemical Co. LTD.
The data from MTT assay showed that CM_EE incubation exerted the significant inhibitory effects on DU145 and PC3 cell proliferation in dose- and time-dependent manner both with IC50= 50 μg/ml. Flow cytometry with PI staining demonstrated that DU145 and PC3 cells were arrested in cell cycle G2/M-phase in presence of CM_EE. Flow cytometry with annexin V/PI staining indicated that CM_EE could induce apoptosis in CM_EE-incubated DU145 and PC3 cells. The results from western immunoblotting analyses, which was hybridized with antibodies against various effector proteins associated with cell cycle and apoptosis, demonstrated that CM_EE treatment inhibited the expressions of CDK1, cyclin B1, Cdc25C, P-Cdc25C, Aurora A, Aurora B. Showed that increase the expression of caspase-3, -8 with -9.
The ingredients present in C. militaris fruit body might be a potential source of chemoprevention for prostate cancer.
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