| Summary: | 碩士 === 南臺科技大學 === 生物科技系 === 104 === The medicinal Inonotus obliquus (I. obliquus) is a white rot fungus, and it inhabits on birch trees in the birch forests of Russia, Eastern Europe, China, Japan. The sclerotia have been used as a folk medicine since the 16th century, and have exhibited various biological activities, including anti-tumor, anti-viral, anti-oxidation.
In this study, four different fractions, hot water extraction (HWE), ethanol extraction (EE), hot water extraction followed by ethanol extraction (HW-EE) and gradient method of ethanol extraction, were evaluated for extraction of the total phenolic content from the solid state fermentation of I. obliquus. The change of the total phenolic content and the antioxidant capacity at the cultural period of the solid state and submerged fermentation were also investigated, respectively.
The results showed that the total phenolic content increased with the cultural time of I. obliquus by solid-state fermentation using brown rice as culture medium. The highest of total phenolic content, 69.4 mg GAE/g, was obtained by gradient method of ethanol extraction at 120 days after inoculation.
The EC50 concentration of ABTS, reducing power and OH radical scavenging activity were 3.84, 0.75and 3.63 mg/ml from the solid-state fermentation, respectively. The EC50 concentrations of ABTS, reducing power and OH radical scavenging activity was10.19, 3.2 and 5.16 mg/ml from the submerged fermentation is using maltose and himedia peptone, respectively.
It showed the antioxidant capacities of the solid state and submerged fermentation of I. obliquus correlated with the total phenolic contents of extracts. The product of solid-state and submerged fermentation of I. obliquus has the potential to be the functional foods.
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