| Summary: | 碩士 === 南臺科技大學 === 生物科技系 === 104 === Spinal cord injury (SCI) is spinal cord and nerves damage by trauma, resulting in motor, sensory and excretory dysfunctions. There are more than 65,000 patients with spinal cord injury in Taiwan nowadays. SCI not only seriously affect victims' personal and family lives, but also cost a lot of resources from community. Current treatments for SCI include medications, medical procedures, functional rehabilitation, gene therapy and other modalities. Unfortunately, those treatments have their limits and there is no real functional recovery experience. Therefore, to find an effective treatment of spinal cord injury is indeed imperative. In recent years, the use of stem cell therapy for spinal cord injury has been widely discussed, is considered to be a viable treatment option. Previous studies have pointed out that mesenchymal stem cell transplantation in the treatment of cells for the treatment of a new model. Human umbilical cord derived mesenchymal stem cells (hUCMSCs), one such candidate with high potential, are a fetus-derived stem cell source collected from discarded tissue (Wharton's jelly) after birth. Compared with human bone marrow derived mesenchymal stem cells, hUCMSCs have the advantages of abundant supply, painless collection, no donor site morbidity, and faster and longer self-renewal in vitro. From other researchers studies, hUCMSCs improved hindlimb motor dysfunction of spinal cord injury rats.
Professor Chen Sheng- Hsien and other scholars from the past research that human umbilical cord mesenchymal stem cells improves limb motor dysfunction after SCI in rats. From other researchers studies, hUCMSCs improved hindlimb motor dysfunction of spinal cord injury rats. A laminectomy with removal of the vertebral peduncle, was performed at T8 or T9 on male Sprague-Dawley rats anesthetized with zoletil (25 mg/kg, i.p.) and atropine (0.1 mL, s.c.) The jaws of a calibrated aneurysm clip with a closing pressure of 55 g were placed between the dorsal and ventral surface of the spinal cord and left in place for 1 min. All SD rats are randomly assigned to five groups: (1) N=6; sham control; (2) N=6; after SCI, 1 cc saline iv immediately and 7 days after SCI, 10μl culture medium transplanted into epicenter of injury; (3) N=6; after SCI, hUCMSCs (2.5×105) in 1 cc saline iv immediately and 7 days after SCI, 10μl culture medium transplanted into epicenter of injury; (4) N=6; after SCI, 1 cc saline iv immediately and 7 days after SCI, hUCMSCs (2.5×105) in 10μl culture medium transplanted into epicenter of injury; (5) N=6; after SCI, hUCMSCs (2.5×105) in 1 cc saline iv immediately and 7 days after SCI, hUCMSCs (2.5×105) in 10μl culture medium transplanted into epicenter of injury; (6) N=6; after SCI, hUCMSCs (2.5×105) in 1 cc saline iv immediately and 7 days after SCI, hUCMSCs (2.5×105) in 10μl culture medium transplanted into epicenter of injury. All rats except for group 6 received cyclosporine A (10 mg/kg, IP) daily from day 5 after SCI till the completion of the experiments. Herein, The Basso, Beattie, and Bresnahan (BBB) locomotor rating scale was used to measure functional recovery of the rats on the first, second and third week after SCI.
After spinal cord injury, in addition to causing animal hindlimb paralysis and dysfunctional behavior, which also occurs after spinal cord injury apoptosis (cell apoptosis) case, it is also more after spinal cord injury, apoptosis in spinal cord tissue and Caspase- 3's performance. Experiment utilizing TUNEL stain (The terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling assay) and immunofluorescence assay of caspase-3 was measured to do.Spinal cord injury can also cause nerve cells to shrink and sheath (demyelination) and other direct injury, spinal cord injury will start from restoration, during which there will be a lot of scar tissue filler (glial scar formation) lesions, interference neuraxis sudden regeneration (axonal regeneration) thereby affecting nerve conduction. GFAP stain from the results that, given the continuous implantation of spinal cord injury between human umbilical cord mesenchymal stem cell therapy and Sham, saline + CM group and Saline + hUC-MSCs compared to some significant improvement in the injured spinal cord tissue glial scar formation .
Therefore, we investigate therapeutic effect of human umbilical cord derived mesenchymal stem cells sequential transplantation after experimental SCI.
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