The studies on antioxidant and hepatoprotective effects of water extract of Lonicera japonica Thunb.

• Main Authors: HSIEH, TING-CHE, 謝定哲
• Other Authors: SHIEH, DEN-EN
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/q43w79

碩士 === 大仁科技大學 === 食品科技研究所 === 104 === According to the statistics of the Ministry of Health and Welfare in 2014, the chronic hepatitis and cirrhosis ranked the ninth on the ten leading causes of death in Taiwan. Chinese herbal medicine has long been used in the Chinese populated area for treatment of liver illness, and Lonicera japonica Thunb. is one of the popular folk medicine for such purpose. The present work study the in vitro antioxidation and in vivo hepatoprotective effects of the aqueous extract of dried bud of L. japonica Thunb. on carbon tetrachloride-induced acute and chronic hepatitis rat models. Silymarin treated group was used as the positive control group. The result of in vitro antioxidation test indicated that the aqueous extract of L. japonica Thunb.exhibited the greatest antioxidation effect by showing the best DPPH radical scavenging and reduction activity with IC50values of43.4 and 415.4 ppm, respectively. In the treatment of carbon tetrachloride-induced acute hepatitis rat model, the 3-days feeding with low, medium and high dose (50.0, 200.0, 500.0 mg/dl, respectively) of aqueous extract of L. japonica Thunb. resulted in AST values of 209.8 ± 42.4, 232.6 ± 36.1 and 219.8 ± 30.5 U/L, respectively; and ALT values of 295.2 ± 27.0, 303.0 ± 30.0 and 297.4 ± 43.5 U/L, respectively; and blood triglyceride level of 68.5 ± 15.1, 83.8 ± 7.0 and 69.6 ± 22.5 mg/dl, respectively. All the above parameters indicated the attenuation of carbon tetrachloride-induced acute hepatitis in control rat model with average AST value of 526.8±131.8 U/L, ALT value of 522.2 ± 38.0 U/L and blood triglyceride level of 157.5±23.3 mg/dl. Liver biopsies of carbon tetrachloride-induced acute hepatitisrat models with H&Estainingshowed that the liver tissue-repairing ability was improved in the group treated with aqueous extract of L. japonica Thunb. In the study of carbon tetrachloride-induced chronic hepatitis rat model, 8-weeks feeding with low, medium and high dose of aqueous extract of L. japonica Thunb. resulted in AST values of 420.0 ± 6.5 U/L, 623.0 ± 8.8 U/L and 635.3 ± 6.2 U/L, respectively；ALTvalues of 420.0 ± 6.5 U/L, 420.0 ± 7.7 U/L, and 541.8 ± 7.3 U/L, respectively； and blood triglyceride level of 76.4 ± 12.2, 73.0 ± 6.6 and 60.5 ± 4.8 mg/dl, respectively. All these parameters again indicated the attenuation of carbon tetrachloride-induced chronic hepatitis in control rat model with average AST value of 864.5 ± 7.4 U/L, ALT value of 64.8 ± 4.7 U/L and blood triglyceride level of 74.9 ± 6.0 mg/dl. Liver biopsies of carbon tetrachloride-induced chronic hepatitis rat models with H&E staining revealed that the liver tissue-repairing ability was improved with dose-dependent manner in the group treated with aqueous extract of L. japonica Thunb.Liver biopsies of carbon tetrachloride-induced chronic hepatitis rat models with Masson's trichrome staining for collagenous tissue fibers indicated that the collagen proliferation rate in liver was reduced with increasing treatment dosage. Furthermore, in vivo antioxidation test indicated that medium and high dosage of aqueous extract of L. japonica Thunb. increased the liver GPx content ( 1.39 ± 0.19 and 2.01 ± 0.11, respectively; CCl4: 1.33 ± 0.00 U/mg protein ), and significantly inhibited the formation of liver MDA ( 1.52 ± 0.10、 1.03 ± 0.04； CCl4: 1.68 ± 0.05μ mole/mg protein ). Meanwhile, low, medium and high dose of aqueous extract of L. japonica Thunb.significantly increase thecatalase activity in liver ( 50.33 ± 4.89, 56.41 ± 0.29 and 54.99 ± 2.45, respectively； CCl4: 49.94 ± 1.87 U/ mg protein ). To sum up, the aqueous extract of L. japonica Thunb. has demonstrated superior in vitro and in vivo antioxidation effect, and enhanced in vivo liver repairing activity against carbon tetrachloride-induced acute and chronic hepatitis. The action mechanism of its hepaprotective effect deserves further exploration. Keywords: Lonicera japonica Thunb., Carbon tetrachloride, Hepatoprotective effect, Antioxidation