Preparation of vanadium oxide nanorods and thulium(III) hexacyanoferrate modified electrodes for electrochemical sensing of hydrogen peroxide and L-cysteine

• Main Authors: Wei-Lun Cheng, 程偉倫
• Other Authors: Shen-Ming Chen
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/55h57n

碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班（碩士在職專班） === 104 === Part I：Generally speaking, electrochemical sensors for the detection of hydrogen peroxide can be classified into two categories：enzymatic and non-enzymatic. Although these enzyme type sensors with high sensitivity、high selectivity and other characteristics, At the same time , the temperature、humidity and pH affects it. therefore,development of non-enzymatic sensors become a trend. Herein, we report a simple hydrothermal synthesis of V2O5 nanorods employed as electrode materials for non-enzymatic hydrogen peroxide (H2O2) biosensor application. The morphology and structural properties of the as-synthesized material is characterized by various physicochemical and electrochemical methods viz. scanning electron microscope (SEM), Energy Dispersive X-ray Spectroscopy (EDX), X-ray diffraction (XRD), Fourier transform infrared spectra (FTIR), cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Remarkably, an excellent electrochemical performance has achieved by using the as-modified V2O5 nanorods glassy carbon electrode (GCE) towards the reduction of H2O2.Hence, the novel as-constructed V2O5 nanorods electrodes provides a new avenue for the amperometric H2O2 sensor fabrication, suggesting that holds a promising potential in the field of wide electrochemical industrial applications. The V2O5 nanorods modified electrode shows a considerable linear range, sensitivity and detection limit of about 0.1 to 408 µM, 690.6 µA µM-1 cm-2 and 0.1 µM respectively for H2O2. Part II：L-cysteine (L-Cys) is the sulfur containing amino acid with and it act as an antioxidant which may prevent the liver diseases . It has found in many pharmaceutical applications; specifically, it is used in antibiotics, for the treatment of skin damage. The shortage of the L-Cys causes much illness, such as liver damage, and loss of muscle and sever skin problem . Hence, the probing of L-cys in body fluids, pharmaceuticals, and food samples is very significant.We report the preparation of star fruit-like Thulium hexacyanoferrate (TmHCF) by using one-step electrochemical deposition in cyclic voltammetry technique. The electrochemically deposited star fruit-like TmHCF is exhibit unique electrocatalytic activity towards the detection of L-cysteine. The as prepared TmHCF was characterized using different techniques such as SEM、X-ray、EIS. The electrochemical activity of star fruit-like TmHCF was studied using CV, and DPV. The star fruit-like TmHCF modified electrode shows a considerable linear range, sensitivity and detection limit of about 0.5 to 8.92 µM, 48.479 µA µM-1 cm-2 and 16 nM respectively for L-cysteine .