Study of Modified Microtube Array Membranes as Cells Carrier in Encapsulated Cell Therapy

• Main Authors: Chia-Wei Li, 黎家瑋
• Other Authors: Yung-Chin Yang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/mg992d

碩士 === 國立臺北科技大學 === 材料科學與工程研究所 === 104 === Cell microencapsulation technology permits the bidirectional diffusion of molecules such as the influx of nutrients, oxygen, and growth factors. At the same time, the membrane prevents immune cells and antibodies from destroying the encapsulated cells. In this study, polysulfone microtube array membrane (PSF MTAM) was produced by Taipei medical university (Chien-Chung Chens Lab). Highly aligned property and highly specific surface area of MTAM made it a good cells carrier, but it didn’t have good biocompatibility. MTAM was modified by plasma treatment and grafting γ-PGA to improve its hydrophilic. DPPH was used to find the perfect time (90 s) of plasma treatment and FTIR was used to examine that γ-PGA was successfully grafted on MTAM. Water contact angle analysis and wicking test were used to examine the hydrophilic property of MTAM after modifying. Tensile strength of material were analyzed by micro tensile test. Finally, the biocompatibility of MTAM was analyzed by cell proliferation assay with MTS, and cells fluorescent staining to observe the growing situation of MG63 inside encapsulated. In conclusion, grafting γ-PGA on MTAM could promote biomedical characteristic effectively to let MTAM has the potential to be the encapsulated cell therapy carriers.