The Turnover Rates of Marker Substances from Paeonia lactiflora in Traditional Chinese Medicine Decoctions

• Main Authors: Li-Yao Tsai, 蔡麗瑤
• Other Authors: Hsiu-O Ho
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/80141358444859380487

碩士 === 臺北醫學大學 === 藥學系(碩博士班) === 104 === Peony root (PR), one of the most well-known medicinal material, is the peeled and dried root of Paeonia lactiflora Pall. (Fam. Ranunculaceae). According to Taiwan Herbal Pharmacopoeia, paeoniflorin content in PR should not be less than 1.0%. However, there is no standard for the quantitation of analytical markers in PR related concentrated extract preparations (CEPs). In this study, three formulae, Shao Yao Gan Cao Tang (SYGCT), Si Wu Tang (SWT) and Si Ni San (SNS), in which PR serves as a basic ingredient were selected. Four makers - paeoniflorin, albiflorin, benzoic acid and gallic acid were quantitated by HPLC, then computed the turnover rates and concentration rates in all three decoctions. The turnover rates of paeoniflorin, albiflorin, benzoic acid and gallic acid in decoctions were 73.8-86.1%, 77.8-97.6%, 154.7-325.0% and 106.9-177.9% respectively. The concentration rates of paeoniflorin in SYGCT, SWT and SNS were 89.5%, 89.1-101.8% and 88.6-97.9%, respectively. For (1) albiflorin, (2) benzoic acid and (3) gallic acid the concentration rates in SYGCT, SWT and SNS were (1) 89.4%, 82.0-99.0% and 94.6-113.6%, (2) 77.3%, 45.1-97.3% and 90.0-171.6%, (3) 84.6%, 67.6-90.0% and 89.3-96.8%, respectively. Paeoniflorin displayed the lowest concentration rates which indicated the great stability. The followings were albiflorin, gallic acid and benzoic acid. Also, this study found the pH and the contents of phytochemicals of samples varied the concentration rates. The pH significantly altered the concentration rates of benzoic acid and gallic acid, but not albiflorin and paeoniflorin. Total polysaccharides influenced the concentration rates of albiflorin, benzoic acid and gallic acid. Total polyphenols and condensed tannins modified the concentration rates of benzoic acid. Total saponins also changed the concentration rates of benzoic acid and gallic acid. Therefore, the fluctuating concentration rates of albiflorin, benzoic acid and gallic acid made them poor candidates to be selected as quality control markers. Paeoniflorin was thus the most suitable analytical marker for PR related CEPs. In this study, quantitative analysis for marker components in four commercial CEPs (PR, SYGCT, SWT and SNS) were conducted. The results showed benzoic acid significantly fluctuated while albiflorin, benzoic acid and paeoniflorin performed relatively stabilities. SNS CEPs were seen small variations in four markers compared to PR, SYGCT and SWT. According to the turnover rates and content (1.0%) of paeoniflorin in PR regulated in Taiwan Herbal Pharmacopoeia, this study suggested a 70% turnover rate and a 0.5% of paeoniflorin content that could be set as lower limits for the standard of PR related CEPs.