| Summary: | 碩士 === 臺北市立大學 === 應用物理暨化學系 === 104 === We developed a sensor that nanoceria particles (CeO2) act as an efficient quencher and boron dipyrromethane conjugated adenosine 5’-triphosphate (BODIPY-ATP) as fluorophore for sensing analytes containing phosphate group such as pyrophosphate ions(ppi) and adenosine 5’triphosphate(ATP) etc. BODIPY-ATP molecules was chelated on the surface of CeO2 through phosphate group and Ce3+ that as a bridge to induce the photoelectron transfer from BODIPY to CeO2. According to this mechanism, we designed the turn-on fluorescence sensors for detecting various species. For instances, Ppi is one of the product by ATP hydrolysis which could be as an indicator for metabolism. Compared with BODIPY-ATP, the size of ppi is smaller that could compete against BODIPY-ATP to coordinate on the surface of CeO2. Once the ppi concentration is increased, less BODIPY-ATP can bind to CeO2. Consequently, BODIPY-ATP fluorescence increases with increased ppi concentration. Similarly, we also used ATP as a model to illustrate the sensing strategy. The fluorescence increased linearly with increasing ppi and ATP concentration raging from 4 to 60 nM and 40 to 620 nM, respectively. The probe’s detection limits at a signal-to-noise ratio of 3 for both ppi and ATP were estimated to be 1.5 nM and 15 nM, respectively. In addition, Alkaline phosphatase (ALP) is an important hydrolysis enzyme in human body. We proposed the ALP sensor by the ALP-catalyzed hydrolysis of BODIPY-ATP resulted in the formation of BODIPY-adenosine and phosphate ions. The newly formed BODIPY-adenosine lack of triphosphate that can’t bond to CeO2. CeO2 are incapable of triggering the fluorescence quenching of BODIPY-adenosine. Consequently, the fluorescence of BODIPY-adenosine increase with increasing ALP concentration. The proposed method with linear range was 0.0004~0.00625 Unit/L, and the detection of limit was estimated to be 0.00015 Unit/L. In the future, the practicality of the proposed probe would be demonstrated by quantifying ATP in a single drop of blood.
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