Detection of sodium thiocyanate in milk using silver nanoparticle decorated/embedded anodic alumina SERS substrates

• Main Authors: Hong-Kai Zhang, 張閎凱
• Other Authors: Chi-Hung Lin
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/59751302215866687027

碩士 === 國立陽明大學 === 生醫光電研究所 === 104 === In the past, sodium thiocyanate was added into raw milk as a methods of preservation in the area lacking cooling equipment. However, recent researches indicated over intake of sodium thiocyanate could induce clinical symptoms like nausea, diarrhea, and stomach ache. Therefore, sodium thiocyanate had been banned as additive in dairy products in many countries currently. Established methods to detect sodium thiocyanate include ion chromatography (IC), high performance liquid chromatography (HPLC), mass-spectrography and more, but those methods take much time for pretreatment and detection. In our research, Surface-enhanced Raman spectroscopy (SERS) was used for rapid detection of sodium thiocyanate in milk using silver nanoparticle embedded anodic alumina oxide (AgNPs/AAO) substrate. The limit of detection (LOD) for sodium thiocyanate was 0.02 mg/L in water. The AgNPs/AAO substrate represented good reproducibility and stability. A good linear relationship was obtained for sodium thiocyanate in water at concentration from 0-81 mg/L. The simple pretreatment procedures and analytical methods are less time consuming and make a rapid method for detection of sodium thiocyanate.