酵素薄膜之潤濕梯度微接觸印刷製程可行性研究

• Main Authors: CHEN, WEI-TING, 陳威廷
• Other Authors: CHENG, JUNG WEI
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/zfxr78

碩士 === 國立中正大學 === 機械工程系研究所 === 105 === The purpose of this paper is to extend the microcontact printing technology from the optoelectronics to the biosensors. This paper uses microcontact printing technology combined with the principle of wetting gradient to produce patterned films from enzyme-like solution (no enzyme, but fluid behavior is similar to enzymes) and subsequently transfer the films to the substrate of interest. This not only extends application scope of the microcontact printing but also reduces the waste of expensive enzyme materials. This paper will derive the principle of wetting gradient patterning. And explains the movement of the liquid in different situations. The size of the patterned area is estimated by calculating the volume of the liquid. Since the patterned thin film is easily affected by evaporation. So it will take into account the evaporable issues, and explore the impact of evaporation on the dip coating. In the experiment will encounter the pattern can not be film, pattern deformation and pattern size of the problem. After pulling speed and environmental humidity control has been able to achieve a more stable coating. In the experiment of patterned film transfer, the patterned film can be successfully transferred to glass. But in the transfer to the carbon substrate will encounter the problem of uneven substrate lead to transfer failure. Surface of the carbon substrate after planarizing significantly improved. But only the part of the film transfer to the carbon substrate. At present, this technology has successfully coated a circular patterned films with a diameter of 2.2 mm which successfully been transferred onto a glass substrate. The area ratio between printing result and coating result has reached more than 90%. Keywords : Wetting gradient, Enzyme, Microcontact printing