Development of Biodegradable Shell-core Structured Microspheres for Drug Delivery
碩士 === 長庚大學 === 機械工程學系 === 105 === In this study, electrospraying techniques were used to prepare biodegradable particle carriers. Poly (lactide-co-glycolide), hydrochloride, vancomycin hydrochloride, ceftazidime hydrate, green fluorescent protein, along with different kinds of solvents were used to...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2017
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| Online Access: | http://ndltd.ncl.edu.tw/handle/4b2awg |
| Summary: | 碩士 === 長庚大學 === 機械工程學系 === 105 === In this study, electrospraying techniques were used to prepare biodegradable particle carriers. Poly (lactide-co-glycolide), hydrochloride, vancomycin hydrochloride, ceftazidime hydrate, green fluorescent protein, along with different kinds of solvents were used to manufacture the biodegradable drug-eluting microspheres. The diameter of the microspheres of the solid microspheres and the shell-core microspheres prepared by using the optimum processing parameters is about 5 ~ 10 μm. The microspheres of the shell and core structure were confirmed by transmission electron microscopy and laser scanning confocal microscopy, which proved that the shell - core structure and the fluorescent protein activity of the core layer existed. The confocal images also demonstrated the activity of the fluorescent protein from being damaged by the solvent.
The experimental results showed that electrosprayed microspheres can release antibiotics in vitro for 21 days with concentrations well above the minimum inhibitory concentrations. In vivo experiments with animals also suggested that microsphere can release high drug concentrations (above the minimum inhibitory concentration) over 14 days in the rabbit knee joint. In addition, the experimental results in this study show that microspheres of different size and geometry can be prepared by electrospray technology by changing the processing parameters. This might find potential applications in medicine in the future.
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