Microfluidic three-dimensional focusing device for fluorescent bead detection

• Main Authors: Ching Hui Chang, 張經慧
• Other Authors: Y. H. Lin
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/spk6fz

碩士 === 長庚大學 === 醫療機電工程研究所 === 105 === Since two-dimensional flow-focusing cannot focus particles or cells in vertical(Z-axis) direction, particles are overlapping and out of focus when they are detected in an optical system. Therefore, it is difficult to analyze single particle or cell in accuracy. To solve the above problems, the study proposed a microfluidic three-dimensional focusing device with simple manufacturing characteristics by assembly of coaxial glass capillary. The device was made of one square and two round glass capillaries. One of the round glass capillary was pulled by a micropipette puller so that it had a tapered end. The flow cytometry used calibration fluorescent beads and cells stained with fluorescent were used to test the 3D flow-focusing device. Experimental data showed that the proposed device can focus the beads and cells in a 10 μm width fluid stream and the number of particles and the intensity of fluorescent signal can be detected by a photomultiplier tube. The study looks forward to providing a device with low-cost, easy-to-manufacture and rapid detection for biomedical application.