| Summary: | 碩士 === 中央警察大學 === 鑑識科學研究所 === 105 === According to Art. 191 of CRIMINAL LAW and Art. 49 of RULES OF THE ACT GOVERNING FOOD SANITATION, if acts are served and may
be sufficient to harmful to human health, imprisonment or fine may be imposed; based on Art. 15 of RULES OF THE ACT GOVERNING FOOD SANITATION, Foods or food additives shall not be those with pesticide
residue exceeding the permissible tolerance. As a result, it’s necessary to
develop Pesticide Residue Analysis (PRA) in order to solve cases concerning
food forensics.
In this study, 205 pesticides were seperately subjected to LCQTOF/MS giving rise to the corresponding ESI-TOF/MS and ESITOF/MS/MS mass spectrums libraries. We take isobaric and isomeric pesticides for example to elucidate the importance of these libraries, and introduce the application of All ions MS/MS technique as well. The QuEChERS and d-SPE method was used for multi-residue pesticides in
vegetable samples and was gone through validation. Calibration curves of 170
pesticides were linear (R^2 > 0.99) and mostly covered two orders of magnitude (from 1 to 200 ng/mL). LOD and LOQ ranged from 1 to 10 ppb in lettuce-matrix. Recoveries of 157 pesticides ranged between 70~120%. The Intra-day and Inter-day CVs of 150 pesticides were mostly below 20%. Matrix effect of 149 pesticides spanned ±20%. Market samples trials、crop field trials and wash-off trials were performed to demonstrate the applicability of the method in routine analysis.
This study shows the potential of LC-QTOF/MS for qualitative and quantitative multi-residue analysis of pesticides residue in vegetables. It serves as a guide for those working in food forensics field of pesticides residue analysis, which is important in protecting consumers’ health and enforcing food law.
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