Effects of Extracts From Phyllostachys makinoi on Inhibitory Mechanisms of Staphylococcus aureus and Skin Wound Healing

• Main Authors: WU, JU-YUN, 吳如芸
• Other Authors: TSAI, TSUNG-YU
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/37vqhr

碩士 === 輔仁大學 === 食品科學系碩士班 === 105 === Phyllostachys makinoi is an endemic species in Taiwan and is accounted for 24.92% of the bamboo forest. Bamboo leaves and shoots have shown to possess biological activities such as antioxidant, antiinflammatory and antibacterial activity. The objectives of this study were to investigate the antimicrobial activities and mechanisms of extracts from P. makinoi on non-antibiotic resistant Staphylococcus aureus BCRC 10451 and methicillin-resistant S. aureus BCRC 15211 and also understand the antiinflammatory activity and the effects on skin wound healing. As the results, the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) values of water extracts against both S. aureus strains were 4 and 16-32 mg/mL, respectively. The MIC and MBC values of ethanol extracts against both strains were 2 and 32 mg/mL, respectively. By scanning electron microscopy, bacterial cells treated with both extracts became rough, ruptured and shriveled. In addition, extracts exert their inhibitory effect through the permeability of cell membrane associated with generalized membrane-disrupting effects, leading to the leakage of electrolytes as well as losses of proteins and nucleic acids. These changes resulted in cell decomposition and death eventually. In the assay of antiinflammatory activity, LPS-induced inflammatory RAW 264.7 macrophage cells treated with 100 μg/mL of water extract and 50 μg/mL of ethanol extract can significantly decreased the nitric oxide production and proinflammatory cytokines including tumor necrosis factor-α, interleukin -1β and interleukin-6 content (p < 0.05). Besides these, 600 μg/mL of water extract and 75 μg/mL of ethanol extract can significantly increase the proliferation and migration of CCD-1112Sk human skin fibroblast (p < 0.05). In summary, water and ethanol extracts from P. makinoi revealed the effects on antibacterial and anti-inflammatory activity and proliferation and migration of fibroblast so that had potential on wound infection and wound healing.