Study on the Whitening Mechanism of α-Mangostin From Nutshell of Garcinia Mangostana

• Main Authors: LIAO, CHUN-CHIEH, 廖竣傑
• Other Authors: CHANG, TSONG-MIN
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/86anpm

碩士 === 弘光科技大學 === 化妝品科技研究所 === 105 === α-Mangostin is a type of xanthones compound isolated from Garcinia mangostana. It has several biological including anti-inflammatory, anti-tumor, antidiabetic, antibacterial, antifungal, antiparasitic, antioxidant and anti-obesity activities. The purpose of this study was to investigate the melanogenesis inhibitory properties and antioxidant properties of α-mangostin. Assays of mushroom and intracellular melanin contents and tyrosinase activites were conducted spectrophotometrically while expression profiles of the melanogenesis -related proteins were determined by Western blotting. Further, The possible signaling pathways involved in α-mangostin-mediated inhibition of melangenesis were also survey from specific protein kinase inhibitors. The result from the study indicated that α-mangostin (30-300μM) inhibits mushroom tyrosinase activity in a dose-dependent pattern. The, α-Mangostin repressed α-MSH induced intracellular tyrosinase activity and reduced melanin content in mouse B16F10 melanoma cells . α-Mangostin also suppressed the intracellular reactive oxygen species (ROS) level in a dose dependent matter. The results showed that α-Mangostin inhibited melanogenesis in B16F10 cells via activating PI3K/AKT (Protein kinase B) pathways. And reducing the level of intracellular ROS. Therefore, α-Mangostin could be applied as a natural skin whitening ingredient in skin care products.