Separation and Purification of cis,cis-Muconic Acid from Fermentation Broth

• Main Authors: Ming-Yao Huang, 黃明耀
• Other Authors: Jiumn-Yih Wu
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/74k3xz

碩士 === 義守大學 === 化學工程學系暨生物技術與化學工程研究所 === 105 === In this study, chemical precipitation and ion exchange were used to the recovery of muconic acid from the fermentation broth. To investigate the effects of the experimental conditions on the yield and purity in chemical precipitation treatment, we examined the pH value, the precipitation time and the used weight ratio of the activated charcoal to the precipitate in the operation. The results show that the maximum yield and purity achieved to 85% and 96% while the manipulated pH was at 2.5, the precipitation time was operated more than 30 minutes and the used weight ratio of the activated charcoal to the precipitate was controlled at 12.5%. In order to find the suitable resin for the recovery of muconic acid, we checked the ionic forms of different resin to identify the influence of the inlet muconic acid concentrations and the operating pH values. The results showed that Cl form had more advantages than OH form in experimental operation and the storage of resins. We also found the Amberlite IRA 400 had the highest adsorption capacity of 110 mg muconic acid/g resin in this system. In addition, the muconic acid adsorption behavior by using IRA 400 resin followed the Freundlich isotherm model. Furthermore, Amberlite IRA 400 not only had a stable adsorption capacity at 25℃-35℃, but also had a good selectivity to muconic acid recovery while other organic acids in solution simultaneously. The breakthrough curve was obtained by using Amberlite IRA 400 as an adsorbent and NaCl as a desorbent. After investigation of the different flow rates and the desorbent concentrations, it could be found the adsorption percentage was 44.47% and the desorption percentage was 88% by carrying out a optimal flow rate of 3.84 BV/hr and desorbent concentration of 0.5 M.