Determination of glucosamine and luteolin using disposable screen-printed carbon electrode modified with carbon materials and metal nanoparticles

• Main Authors: Ting-Wen Hu, 胡婷雯
• Other Authors: Yu-Tse Wu
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/3kjg7b

碩士 === 高雄醫學大學 === 藥學系碩士班 === 105 === In this study, working electrodes in an electrochemical system were chemically modified by different kinds of metal nanoparticles and carbon materials. The modified electrodes were used to develop an electrochemical analytical method and practically apply on pharmaceutical samples. The study was divided into two parts, in the first part, copper nanoparticles (Cu NPs) and graphene or carbon dots (CDs) were electrodeposited on screen-printed carbon electrode (SPCE) by the amperometric i-t curve. With the modification, graphene/Cu NPs modified SPCE could detect glucosamine, which was not able to be detected directly. Graphene/Cu NPs modified SPCE was further used to study electrochemical behavior of glucosamine by cyclic voltammetry, and develop an analytical method to determine glucosamine by the amperometric i-t curve. In the second part of the study, palladium nanoparticles (Pd NPs) and fullerene (C60) or CDs were electrodeposited on SPCE by cyclic voltammetry. The modified electrode was used to study electrochemical behavior of luteolin with the same electrochemical method and develop an analytical method to determine luteolin by differential pulse voltammetry. By amperometry with the fixed potential of +0.6 V, the results of the first study of glucosamine determination demonstrated the linear range of the calibration curve was 21000 μM with R2 of 0.9951 and detection limit of 1 μM. In addition, the analytical time was short that it could determine a sample within one minute. Also, it can be directly applied on four glucosamine pharmaceuticals without separation of columns. The recovery study was 95110% with RSD below 6.5% for three products and 5570% with RSD below 7% for one. Moreover, the graphene/Cu NPs modified electrode also exhibited great repeatability (CV = 5.0%), reproducibility (CV = 3.8%), and stability. In the second part of the study, the analyte luteolin was measured with fullerene/Pd NPs modified SPCE by differential pulse voltammetry in the potential range from 0 to +0.8 V for the oxidation current. The linear range of the calibration curve was 0.0525 μM with R2 of 0.9965 and detection limit of 0.01 μM. And fullerene/Pd NPs modified electrode also showed great repeatability (CV = 4.2%) and stability. In conclusion, with the electrochemical method of amperometry, graphene/Cu NPs modified SPCE succeed to determine glucosamine pharmaceuticals directly without separation or derivatization of structure and the analytical performance was accurate and stable. On the other hand, fullerene/Pd NPs modified SPCE were successfully prepared and achieved to determine luteolin effectively, stably, and accurately.