Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings
碩士 === 國立中興大學 === 生命科學系所 === 105 === Salinity increases the level of ROS (reactive oxygen species) and the activities of certain antioxidative enzymes in ice plant (Mesembryanthemum crystallinum L.). The effect of salicylic acid (SA) is still unknown in ice plant under salt stress. In this thesis, I...
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ndltd-TW-105NCHU51050112017-09-15T04:40:21Z http://ndltd.ncl.edu.tw/handle/04233041848022338327 Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings 水楊酸前處理減輕冰花幼苗在鹽逆境下所誘導的氧化傷害 Kai-Yi Huang 黃楷懿 碩士 國立中興大學 生命科學系所 105 Salinity increases the level of ROS (reactive oxygen species) and the activities of certain antioxidative enzymes in ice plant (Mesembryanthemum crystallinum L.). The effect of salicylic acid (SA) is still unknown in ice plant under salt stress. In this thesis, I used nitroblue tetrazolium (NBT) and DAB (3'', 3''-diaminobenzindine) staining to detect the effects of salicylic acid pretreatment on superoxide and hydrogen peroxide content under salt stress. The results showed that pretreatment of SA can reduce the content of superoxide and hydrogen peroxide indicating that SA could reduce oxidative stress in ice plant seedlings under salt stress. To determine which antioxidative enzymes were affected by SA pretreatment, I detected activities of four antioxidative enzymes including APX (ascorbate peroxidase), MDHAR (monodehydroascorbate reductase), DHAR (dehydroascorbate reductase) and CAT (catalase). The results showed that pretreatment of SA enhanced the activity of APX, MDHAR, DHAR and CAT under salt stress, indicating that SA could reduce ROS accumulation under salinity-induced oxidative stress. In addition, the effects of salt stress on the activities of APX, MDHAR, DHAR and CAT were not consistent, suggesting that salt stress had different effects on different antioxidative enzymes. 顏宏真 2017 學位論文 ; thesis 38 zh-TW |
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碩士 === 國立中興大學 === 生命科學系所 === 105 === Salinity increases the level of ROS (reactive oxygen species) and the activities of certain antioxidative enzymes in ice plant (Mesembryanthemum crystallinum L.). The effect of salicylic acid (SA) is still unknown in ice plant under salt stress. In this thesis, I used nitroblue tetrazolium (NBT) and DAB (3'', 3''-diaminobenzindine) staining to detect the effects of salicylic acid pretreatment on superoxide and hydrogen peroxide content under salt stress. The results showed that pretreatment of SA can reduce the content of superoxide and hydrogen peroxide indicating that SA could reduce oxidative stress in ice plant seedlings under salt stress. To determine which antioxidative enzymes were affected by SA pretreatment, I detected activities of four antioxidative enzymes including APX (ascorbate peroxidase), MDHAR (monodehydroascorbate reductase), DHAR (dehydroascorbate reductase) and CAT (catalase). The results showed that pretreatment of SA enhanced the activity of APX, MDHAR, DHAR and CAT under salt stress, indicating that SA could reduce ROS accumulation under salinity-induced oxidative stress. In addition, the effects of salt stress on the activities of APX, MDHAR, DHAR and CAT were not consistent, suggesting that salt stress had different effects on different antioxidative enzymes.
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author2 |
顏宏真 |
author_facet |
顏宏真 Kai-Yi Huang 黃楷懿 |
author |
Kai-Yi Huang 黃楷懿 |
spellingShingle |
Kai-Yi Huang 黃楷懿 Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
author_sort |
Kai-Yi Huang |
title |
Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
title_short |
Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
title_full |
Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
title_fullStr |
Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
title_full_unstemmed |
Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
title_sort |
pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/04233041848022338327 |
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