Pretreatment of salicylic acid on alleviation of salinity-induced oxidative damage in ice plant seedlings

• Main Authors: Kai-Yi Huang, 黃楷懿
• Other Authors: 顏宏真
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/04233041848022338327

碩士 === 國立中興大學 === 生命科學系所 === 105 === Salinity increases the level of ROS (reactive oxygen species) and the activities of certain antioxidative enzymes in ice plant (Mesembryanthemum crystallinum L.). The effect of salicylic acid (SA) is still unknown in ice plant under salt stress. In this thesis, I used nitroblue tetrazolium (NBT) and DAB (3'', 3''-diaminobenzindine) staining to detect the effects of salicylic acid pretreatment on superoxide and hydrogen peroxide content under salt stress. The results showed that pretreatment of SA can reduce the content of superoxide and hydrogen peroxide indicating that SA could reduce oxidative stress in ice plant seedlings under salt stress. To determine which antioxidative enzymes were affected by SA pretreatment, I detected activities of four antioxidative enzymes including APX (ascorbate peroxidase), MDHAR (monodehydroascorbate reductase), DHAR (dehydroascorbate reductase) and CAT (catalase). The results showed that pretreatment of SA enhanced the activity of APX, MDHAR, DHAR and CAT under salt stress, indicating that SA could reduce ROS accumulation under salinity-induced oxidative stress. In addition, the effects of salt stress on the activities of APX, MDHAR, DHAR and CAT were not consistent, suggesting that salt stress had different effects on different antioxidative enzymes.