Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate
碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 105 === Green tea is a favorite beverage in Asia, including TAIWAN. It contains four kinds of catechins which are epicatechin, epigallocatechin, epicatechin-3-gallate, and epigallocatechin-3-gallate, respectively. The latter two are esterified catechins. The stu...
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ndltd-TW-105NCHU51050662017-10-09T04:30:39Z http://ndltd.ncl.edu.tw/handle/95373317186422002332 Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate 探討表兒茶素沒食子酸酯及表沒食子兒茶素沒食子酸酯的抗氧化性 Shu-Tzu Hsu 許淑姿 碩士 國立中興大學 生命科學院碩士在職專班 105 Green tea is a favorite beverage in Asia, including TAIWAN. It contains four kinds of catechins which are epicatechin, epigallocatechin, epicatechin-3-gallate, and epigallocatechin-3-gallate, respectively. The latter two are esterified catechins. The studies were antioxidant activities of ECG and EGCG which were ABTS•+ assay, DPPH assay and superoxide anion radical assay of free radical scavenging effect and 50% scavenging effect of concentration. The IC50 is lower, the scavenging effect is better. The results showed that the scavenging effects of ECG and EGCG were dose-dependent. In ABTS•+ and DPPH assay, the scavenging effects of ECG 20 μM and EGCG 20 μM were similar to those of Trolox 400 μM, Pyrogallol 500 μM or Vitamin C 568 μM. In superoxide anion radical assay, ECG and EGCG with the concentration of 100 to 200 μM were tested with 50% scavenging effect of concentration. The free radical scavenging effects of ECG and EGCG were good in ABTS•+ assay, DPPH assay and superoxide anion radical assay. It will be beneficial for antioxidant, nutrition supplementary, disease treat-ment and aging in the future. Chi-Chien Lin 林季千 2017 學位論文 ; thesis 39 zh-TW |
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碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 105 === Green tea is a favorite beverage in Asia, including TAIWAN. It contains four kinds of catechins which are epicatechin, epigallocatechin, epicatechin-3-gallate, and epigallocatechin-3-gallate, respectively. The latter two are esterified catechins.
The studies were antioxidant activities of ECG and EGCG which were ABTS•+ assay, DPPH assay and superoxide anion radical assay of free radical scavenging effect and 50% scavenging effect of concentration. The IC50 is lower, the scavenging effect is better.
The results showed that the scavenging effects of ECG and EGCG were dose-dependent. In ABTS•+ and DPPH assay, the scavenging effects of ECG 20 μM and EGCG 20 μM were similar to those of Trolox 400 μM, Pyrogallol 500 μM or Vitamin C 568 μM. In superoxide anion radical assay, ECG and EGCG with the concentration of 100 to 200 μM were tested with 50% scavenging effect of concentration. The free radical scavenging effects of ECG and EGCG were good in ABTS•+ assay, DPPH assay and superoxide anion radical assay.
It will be beneficial for antioxidant, nutrition supplementary, disease treat-ment and aging in the future.
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author2 |
Chi-Chien Lin |
author_facet |
Chi-Chien Lin Shu-Tzu Hsu 許淑姿 |
author |
Shu-Tzu Hsu 許淑姿 |
spellingShingle |
Shu-Tzu Hsu 許淑姿 Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
author_sort |
Shu-Tzu Hsu |
title |
Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
title_short |
Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
title_full |
Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
title_fullStr |
Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
title_full_unstemmed |
Investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
title_sort |
investigation on antioxidant activities of epicatechin-3-gallate and epigallocatechin-3-gallate |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/95373317186422002332 |
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