| Summary: | 碩士 === 國立中興大學 === 生物化學研究所 === 105 === Head and Neck Squamous Cell Carcinoma (HNSCC) is one of the most prevalent human malignancies worldwide, with a high morbidity and mortality. The epithelial-mesenchymal transition (EMT) has been reported to be an important program that is often activated during the process of cancer invasion and metastasis. EMT not only confers tumor cells with a distinct advantage for metastatic dissemination, but also it provides those cells with cancer stem cell-like characters for proliferation and drug resistance. Luteolin, a flavone found in some vegetables, has been reported to exhibit antioxidant, antiinflammatory, and anticancer activities. In this study, we aimed at investigating the effect of luteolin on epithelial mesenchymal transition induced by TWIST in head and neck squamous cell carcinoma. Herein, we demonstrated that luteolin inhibits TWIST induced mesenchymal phenotype in FaDu cells. Luteolin directly inhibited TWIST1 mRNA and protein expression, upregulated E-cadherin mRNA and protein expression, and downregulated N-andherin, vimentin, Snail and Slug protein expression. Moreover, luteolin inhibited TWIST-induced cell migration and invasion. Luteolin exhibited the inhibitory effects on the TWIST-promoted tumour-initiating abilities in FaDu cells. Luteolin treatment significantly suppressed the TWIST-induced phosphorylation of Akt, GSK-3β and total β-catenin in FaDu-pCDH-TWIST cells. Luteolin treatment significantly suppressed the TWIST-induced upregulation of β-catenin in FaDu-pCDH-TWIST cells .We also found that luteolin inhibited TWIST-induced EMT, migration and tumour-initiating abilities by inhibiting the β-catenin and Akt signalling pathways. Therefore, luteolin could be a potential therapeutic drug for HNSCC.
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