The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells
碩士 === 國立中興大學 === 生物醫學研究所 === 105 === Cellular senescence is a permanent cell growth arrest, and thought a strategy to prevent tumors in vivo. Imiquimod (IMQ) is a ligand of toll-like receptor 7/8 (TLR7/8), contains anti-tumor and anti-viral activity in vitro and in vivo. In our previous studies we...
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ndltd-TW-105NCHU51140032017-09-15T04:40:21Z http://ndltd.ncl.edu.tw/handle/02473464394051705919 The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells 探討 p53 在 Imiquimod 誘導的皮膚腫瘤細胞老化所扮演的角色 Wen-Chien Liao 廖文倩 碩士 國立中興大學 生物醫學研究所 105 Cellular senescence is a permanent cell growth arrest, and thought a strategy to prevent tumors in vivo. Imiquimod (IMQ) is a ligand of toll-like receptor 7/8 (TLR7/8), contains anti-tumor and anti-viral activity in vitro and in vivo. In our previous studies we had demonstrated IMQ induced autophagy and apoptosis in dental doses, but low dose IMQ induced human basal cell carcinomas (BCC) growth arrested and change of morphology. That the characteristic of senescence. Here, we investigated low dose of IMQ (10μg/ml) induced p53-wildtype BCC into senescence. Although, in p53-mutant and p53-knockdown skin cancer cells IMQ might just induce G1 phase arrested and this should have more discussion. We found the role of IMQ-induced senescence in BCC model had interaction with IMQ-induced autophagy. Interruption of autophagy by autophagy inhibitors could also reduce IMQ-induced senescence. Interleukin-6 (IL-6) is an important cytokine of senescence-associated secretory phenotype (SASP). The blockage of IL-6 could reduce IMQ-induced senescence and autophagy. Reactive oxygen species (ROS) induced senescence through DNA damage response (DDR) to activated ATM/ATR pathway was reported. In our study, we eliminated ROS in BCC reduced IMQ-induced senescence. We considered these findings could be useful to basic research and clinical application of IMQ in future. Jeng-Jer Shieh 謝政哲 2017 學位論文 ; thesis 58 zh-TW |
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碩士 === 國立中興大學 === 生物醫學研究所 === 105 === Cellular senescence is a permanent cell growth arrest, and thought a strategy to prevent tumors in vivo. Imiquimod (IMQ) is a ligand of toll-like receptor 7/8 (TLR7/8), contains anti-tumor and anti-viral activity in vitro and in vivo. In our previous studies we had demonstrated IMQ induced autophagy and apoptosis in dental doses, but low dose IMQ induced human basal cell carcinomas (BCC) growth arrested and change of morphology. That the characteristic of senescence. Here, we investigated low dose of IMQ (10μg/ml) induced p53-wildtype BCC into senescence. Although, in p53-mutant and p53-knockdown skin cancer cells IMQ might just induce G1 phase arrested and this should have more discussion. We found the role of IMQ-induced senescence in BCC model had interaction with IMQ-induced autophagy. Interruption of autophagy by autophagy inhibitors could also reduce IMQ-induced senescence. Interleukin-6 (IL-6) is an important cytokine of senescence-associated secretory phenotype (SASP). The blockage of IL-6 could reduce IMQ-induced senescence and autophagy. Reactive oxygen species (ROS) induced senescence through DNA damage response (DDR) to activated ATM/ATR pathway was reported. In our study, we eliminated ROS in BCC reduced IMQ-induced senescence. We considered these findings could be useful to basic research and clinical application of IMQ in future.
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author2 |
Jeng-Jer Shieh |
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Jeng-Jer Shieh Wen-Chien Liao 廖文倩 |
author |
Wen-Chien Liao 廖文倩 |
spellingShingle |
Wen-Chien Liao 廖文倩 The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells |
author_sort |
Wen-Chien Liao |
title |
The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells |
title_short |
The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells |
title_full |
The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells |
title_fullStr |
The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells |
title_full_unstemmed |
The Study of p53 in Imiquimod-Induced Cellular Senescence in Skin Cancer Cells |
title_sort |
study of p53 in imiquimod-induced cellular senescence in skin cancer cells |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/02473464394051705919 |
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