Development of A Bivalent Vaccine against Infectious Bursal Disease and Avian Influenza in Chickens

• Main Authors: Hsiao-Yun Chen, 陳筱云
• Other Authors: Ming-Kun Hsieh
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/82073718090847119934

碩士 === 國立中興大學 === 微生物暨公共衛生學研究所 === 105 === Infectious bursal disease (IBD) and avian influenza (AI) are highly contagious avian viral diseases which cause severe economic loss in poultry industry. Current researches showed that virus-like particles (VLP) of infectious bursal disease (IBDV) has been proved as a safe and efficacious vaccine against IBD. Besides, the VLP of IBDV can also play a role as a vector to carry a foreign protein to form a bivalent vaccine. The purpose of this study is to develop a bivalent vaccine against infection of IBDV and AIV. DNA vaccines are a powerful means of inducing humoral and cellular immune responses and chosen for this study to form IBDV VLP carrying HA protein of AIV. Partial VP3 gene of IBDV was substituted with HA1 gene of AIV (H6N1) and the VP243-HA1 gene was cloned into pTriEx-3 vector, named as pTri/VP243-HA1. The plasmid pTri/VP243 carrying VP243 gene and pTri/HA1 carrying HA1 gene were also constructed as control plasmids. Those plasmids were verified by immunofluorescence assay and western blotting. Purified plasmids and proteins expressed by Escherichia coli expression system and HEK-293T cells were prepared for animal experiment. The result indicated that chickens vaccinated with DNA vaccines and boosted with DNA vaccines or subunit vaccines showed protection against IBDV challenge and positive antibodies against HA1 by ELISA.