| Summary: | 碩士 === 國立成功大學 === 微生物及免疫學研究所 === 105 === Trichomonas vaginalis, an anaerobic protozoa, is one of the most common non-viral sexually transmitted diseases in human. The transmission way for T. vaginalis is typically transmitted through vaginal, oral, or anal sex with an infected individual. It can also be passed from a mother to her baby at birth. The WHO has estimated that 248 million cases of infection was acquired worldwide in 2011, and there are 3 million new cases in the US in 2015. About 70% of infected people do not have any signs or symptoms, but women with trichomoniasis may notice itching, burning, redness or soreness of the genitals, discomfort with urination, or excessive discharge. In this study we created the mimic condition for neutrophil-like (dHL-60) cells and T. vaginalis in a same culture condition, which our medium (DMEM-YIS) supported the two microorganism which are neutrophil (dHL-60) cells and T.vaginalis. Furthermore, in our synthetic medium neutrophil-like (dHL-60) cells and T. vaginalis went to a reaction, which means that T. vaginalis stimulated the neutrophil-like (dHL-60) cells to produce the cytokines responses in our synthetic medium, and also, T. vaginalis produced the SOD6 during the co-incubation.
In this study we have been showed that our synthetic medium which is DMEM-YIS could support the two microorganism for surviving, and also, T. vaginalis could stimulate the neutrophil-like cells to produce the cytokines such as IL-1 and IL-8.
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