Proteomics Analysis to Identify the Metabolic Signatures of Liver and Brown Adipose Tissue in Ovariectomized Rats

• Main Authors: CHIU, YEN-SHUO, 邱彥碩
• Other Authors: HUANG, CHI-CHANG
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/78612585245535223185

博士 === 國立體育大學 === 運動科學研究所 === 105 === Title: Proteomics Analysis to Identify the Metabolic Signatures of Liver and Brown Adipose Tissue in Ovariectomized Rats Abstract: Postmenopausal women are particularly at increased risk of developing non-alcoholic fatty liver disease (NAFLD). Here we aimed to determine the impact of postmenopausal-induced NAFLD (PM-NAFLD) in an ovariectomized rat model. Sixteen six-week-old Sprague-Dawley female rats were randomly divided into two groups (eight per group), for sham-operation (Sham) or bilateral ovariectomy (Ovx). Four months after surgery, indices of liver damage and liver histomorphometry were measured. Both serum aspartate aminotransferase (AST) and alanine aminotranferease (ALT) levels were significantly higher in the Ovx than Sham group. We performed quantitative LC-MS/MS-based proteomic profiling of livers and brown adipose tissues (BAT) from rats with PM-NAFLD to provide baseline knowledge of the PM-NAFLD proteome and to investigate proteins involved in PM-NAFLD by ingenuity pathways analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. For livers, 59 proteins (10.0%) and 48 proteins (8.2%) of the 586 identified proteins were significantly higher and lower, respectively, in the Ovx group compared to the Sham group. For BAT, 5 proteins (0.9%) and 29 proteins (5.2%) of the 554 identified proteins were significantly higher and lower, respectively, in the Ovx group compared to the Sham group. In conclusion, the changes in regulation of proteins implicated in PM-NAFLD may affect other vital biological processes in the body apart from causing postmenopause-mediated liver and BAT dysfunction. Our quantitative proteomics analysis may also suggest potential biomarkers and further clinical applications for PM-NAFLD.