Improving the Real-Time Bio-Sensing Tip for Nitric Oxide

• Main Authors: Chen, Yu-An, 陳俞安
• Other Authors: Meng, Hsin-Fei
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/b734u5

碩士 === 國立交通大學 === 物理研究所 === 105 === In the period of experiment, we make use of the permeability of poly-HEMA (Poly(hydroxyethyl methacrylate)) which covers with the probe of our Nitric oxide sensing tips, Diaminoanthraquinone (DAQ). We use fibers to transfer the signal of the light source, 525nm LED. After the probe reacts with Nitric oxide, the absorbance of the sensing film will change. Because the variation of the intensity of light source is related with the concentration with Nitric oxide, we can extrapolate the amount of Nitric oxide which release in organism, in the future. In order to detect the organism by our sensing tips in the future, we try to change the solution of the sensing films. We replace the solution which is biologically toxic with 95% Ethanol and detect Nitric Oxide successfully. Besides, we try to detect the Nitric oxide of RAW264.7 induced by LPS(Lipopolysaccharide). We find that the response of the sensing tips will change when we only change the thickness of the sensing films so that we use two methods to fix it, that is, by use of the 3D printer and the Teflon moulds. In addition, we try to improve the stability of the system and there are three main works: fixing the device, fixing the sensing tips and changing the original system to potable system. With the improvement of Nitric oxide sensing tips and the system of experiment, we would be more suitable for the detection in organism.