Pluripotency and Differentiation Ability of Human Adipose-Derived Stem Cells Culture in 2-D and 3-D culture

• Main Authors: Chao-Wen Hsieh, 謝肇文
• Other Authors: Akon Higuchi
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/yqfux2

碩士 === 國立中央大學 === 化學工程與材料工程學系 === 105 === Human adult stem cells, such as human adipose-derived stem cells, are considered to be an attractive source of stem cells than human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). This is because human adult stem cells do not generate the ethical concerns that accompany in hESCs. Although human adipose-derived stem cells (hADSCs) are promising for use in regenerative medicine, their lower expansion ability (aging problem) due to the lower pluripotency of hADSCs compared with hESCs and hiPSCs is a critical issue. It’s found that the pluripotency gene expression of Oct4, Sox2, and Nanog in hADSCs after cultivation on TCPS dramatically decreased compared to those in the cells in stromal vascular fraction (SVF) as well as the cells in 3-D culture. There are high pluripotent stem cells in SVFs and 3-D culture, although SVF has more heterogeneous population compared to hADSCs cultured on tissue culture polystyrene (TCPS) dishes. It’s evaluated whether hADSCs can be explained by “Stochastic model” or “Elite model” by hADSCs culture in 2-D culture and 3-D culture sequentially. Precisely, we evaluated the difference of gene expression of the cells when hADSCs were cultured in the 2-D condition (TCPS) and in 3-D condition (ultra low attachment dish) sequentially. It is found that the pluripotent gene expression of hADSCs (Oct4, Sox2 and Nanog) in suspension (3-D culture) is higher than that on TCPS (2-D culture). Especially, low pluripotent gene expression of hADSCs cultured on TCPS is changed to be higher pluripotent gene expression after hADSCs are shifted in 3-D culture