Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells

• Main Authors: Yi Chen, 陳毅
• Other Authors: Yung-Hsi Kao
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/59050782526665764073

碩士 === 國立中央大學 === 生命科學系 === 105 === The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels.