Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells
碩士 === 國立中央大學 === 生命科學系 === 105 === The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this...
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ndltd-TW-105NCU051050092017-04-29T04:32:05Z http://ndltd.ncl.edu.tw/handle/59050782526665764073 Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells 綠茶表沒食子酸酯型兒茶素酸酯在3T3-L1脂肪細胞分化過程中調節FoxO家族基因的表現 Yi Chen 陳毅 碩士 國立中央大學 生命科學系 105 The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels. Yung-Hsi Kao 高永旭 2017 學位論文 ; thesis 58 zh-TW |
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碩士 === 國立中央大學 === 生命科學系 === 105 === The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels.
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Yung-Hsi Kao |
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Yung-Hsi Kao Yi Chen 陳毅 |
author |
Yi Chen 陳毅 |
spellingShingle |
Yi Chen 陳毅 Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells |
author_sort |
Yi Chen |
title |
Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells |
title_short |
Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells |
title_full |
Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells |
title_fullStr |
Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells |
title_full_unstemmed |
Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells |
title_sort |
green tea epigallocatechin gallate regulates the expression of the foxo family during differentiation of 3t3-l1 fat cells |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/59050782526665764073 |
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