Effects of secreted aspartyl proteinase 9 (sap9) onnutrient acquisition in Candida albicans
碩士 === 國立高雄師範大學 === 生物科技系 === 105 === Candida albicans belongs to a part of the normal microflora and a common opportunistic pathogen of human. The Secreted aspartyl proteinases (Saps) produced by C. albicans are considered to be putative virulence factors for candidiasis. The Secreted aspartyl prot...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2017
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| Online Access: | http://ndltd.ncl.edu.tw/handle/37958282841118889127 |
| Summary: | 碩士 === 國立高雄師範大學 === 生物科技系 === 105 === Candida albicans belongs to a part of the normal microflora and a common opportunistic pathogen of human. The Secreted aspartyl proteinases (Saps) produced by C. albicans are considered to be putative virulence factors for candidiasis. The Secreted aspartyl proteinases-9 (Sap9) is a GPI-anchored cell membrane protein. Some research revealed that the Sap9 has a significant effect on cell surface integrity of C. albicans and the putative substrate of this proteinase might be the proteins on its own surface. Our study has found that the hypha-formation of SAP9- deleted mutant (Δsap9) is faster than the wild type strain when they were cultured on solid rich media, suggesting the sensing or acquisition of nutrient may be defect in Δsap9 strain. We further identify that the levels of nitrogen source has a significant effect on the growth of Δsap9 strain. Moreover, we detect the variance of the expression levels of some nitrogen-signaling pathway important molecules between Δsap9 and wild type strains by reverse transcription- polymerase chain reaction (RT-PCR) analysis to further investigate the function of Sap9 in nitrogen sensing and acquisition. This might be a potential aspect to develop therapeutic strategies of candidiasis.
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