| Summary: | 碩士 === 國立屏東科技大學 === 生物科技系所 === 105 === Nervous necrosis virus (NNV) is a bipartite single-stranded positive-sense RNA molecule virus, which caused severe nervous necrosis disease in many kinds of grouper species. In the present study medaka liver cell line OLHE-131 and medaka fish were used as models to study the effect of cinnamaldehyde on the antiviral activity against RGNNV infection. Cytotoxicity assay showed that 5 µg/mL and 10 µg/mL were the appropriate concentration for treating OLHE-131, and the mRNA expressions level of immune-related genes such as IFN-α, IFN-d, MX, ISG15, ISG56, GIG1-α were increased in the presence of 5 µg/mL and 10 µg/mL of cinnamaldehyde. Based on the morphology assay, 5µg/mL of cinnamaldehyde can prevent NNV-induced CPE suggesting cinnamaldehyde potential exhibit antiviral activity against NNV infection. Dietary supplementation with cinnamaldehyde (60 mg/kg) in adult medaka for one month revealed the immune-related genes such as IFN-α, IFN-d, MX, ISG15, ISG56, GIG1-α were significantly increased suggesting cinnamaldehyde possess immunomodulatory functions and potential is an effective immunostimulatory against NNV virus in aquaculture.
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