| Summary: | 碩士 === 國立屏東科技大學 === 食品科學國際碩士學位學程 === 105 === Tournefortia sarmentosa Lam. (Boraginaceae), a Chinese herbal medicine, is commonly used as a detoxicant, anti-inflammatory agent and dermatological medicine. Previous study shows that T. sarmentosa extract can enhance the activities of antioxidant enzyme to prevent low-density-lipoprotein (LDL) peroxidation and anti-acetaminophen-induced hepatotoxicity. Furthermore, the phenolic compounds from Tournefortia sarmentosa was determined and showed the strong activity of antioxidation. Solar radiation is an environmental assault. It is a stress factor and will accelerate aging process of skin. The ultraviolet B (UVB) in the solar radiation can damage DNA directly. It will lead to the DNA mutagenesis, reduce collagen and cause higher risk of skin cancer. The aim of the present study was to investigate the protective effect of the aqueous extract of T. sarmentosa on keratinocytes (HaCaT) and dermal fibroblasts from UVB. Hydrogen peroxide (H2O2) is known as a strong oxidant. It will be taken as a comparison in this study. According to the results, T. sarmentosa extract is harmless to human skin cells and protects them from damages caused by UVB and H2O2. It shows that protective effect of T. sarmentosa extract is related to reduce reactive oxygen species and inhibit activator protein-1 (AP-1) which will cause the collagen breakdown.
Keywords: Tournefortia sarmentosa, Ultraviolet B, Keratinocytes, Dermal fibroblasts, Activator protein-1
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