Effects of Cryoprotectant Composition on the Semen Quality of Frozen-Thawed Canine Semen

• Main Authors: Huang, ruei-chang, 黃芮昌
• Other Authors: Shen, Perng-Chih
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/a5hwad

碩士 === 國立屏東科技大學 === 動物科學與畜產系所 === 105 === Cryopreservation process produces significant changes in mammalian spermatozoa with the subsequent decrease in its fertility potential. Different factors are involved in the alteration of sperm during the cryopreservation: the cryoprotectant used and chilling, freezing and thawed processes employed. Therefore, a large number of extenders have been studied in order to preserve the canine semen quality during the cryopreservation. This study was investigated to explore the effect of glycerol (G), low density lipoprotein (LDL) and trehalose concentration on post-thawing canine semen quality. Semen were collected from three mature Maltese canine (2 and 5 years of age)；The aim of this study is to evaluate the semen quality and is divided into motility (%), progressive (%), viability (%), acrosome integrity (%), mitochondrial integrity (%), and DNA integrity. Results showed that the final glycerol concentration of 5% of the cryoprotectant is significantly higher (P <0.05) than other extender is 3%, 4%, 6% and 7% glycerol concentration in 11% lactose with 20% egg yolk basic extender. When the extender added 11% LDL, canine semen quality (P < 0.05) is significant higher than 10%, 12%, 13% and control group (20% egg yolk) in 11% lactose with 5% glycerol-based extender. Based on the 11% LDL with 5% glycerol based extender, the final optimal trehalose concentration of canine semen cryoprotectant is 350 mM which is better than 300 mM and the control group (11% lactose). In conclusion, the best concentration of glycerol, LDL and trehalose for freezing canine semen are 5 %, 11% and 350 mM. Keywords: Canine, Cryoprotectation, low density lipoprotein, Glycerol, Semen quality, Trehalose