Identifying specific and differentially linked glycosyl residues in mammalian glycans by targeted LC-MS analysis

• Main Authors: Huang, Yi-Li, 黃義澧
• Other Authors: Chen, Sung-Fang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/f3j6mx

碩士 === 國立臺灣師範大學 === 化學系 === 105 === Glycan is a compound consisting a large number of monosaccharides linked glycosidically. Due to its high complexity of glycan structures on glycoproteins, assessing the configuration and position of glycosidic linkages of a glycan is in great demand. In this study, a method via partially O-methylated alditols (PMAs) from glycan combined with LC-MS analysis is developed. N-glycans were first per-methylated with methyl iodide, and levels of methylation were further confirmed with MALDI-TOF. PMAs were then produced via totally hydrolysis and reduction. PMAs from Fetuin N-glycan and Lewisa were successfully detected by LC-MS analysis. In conclusion, this proposed glycan linkage analysis can be performed without additional derivatization step for GC analysis, and should be suitable for the LC-MS-based platform.