The Effect of Four Microalgae Species on the Development, Growth and Survivals of the Larvae of Amano Shrimp (Caridina multidentata) under Two Temperature conditions

• Main Authors: Jude Pean, 朱培安
• Other Authors: Huang, Yii-Shing
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/2fr69a

碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 105 === ABSTRACT The objectives of this study were to evaluate four different algae species, Tetraselmis chuii, Isochrysis galbana, Thalassiosira weissflogii, Chaetoceros muelleri as live feed fed to the Amano shrimp (Caridina multidentata) larvae at equal density of 2 x105cells/ml (three replicates) under two temperature conditions on the development, growth and survivals. A triplicated 4x2 factorial experiment was designed. The newly released shrimp larvae (Zoea1) obtained from pouched brooders were placed in 100 L Fiberglass reinforced plastics (FRP) at a density of 30 larvae/liter and a salinity of 20‰ for each experimental unit. Of the survival rate through covariance analysis, there existed no temperature effect between 29°C and 25°C, with the respective values of 9%, 12%, 35%, 36% for the larvae received C. muelleri, I. galbana, T. chuii, T. weissflogii at high temperature condition (29°C) compared to the corresponding values of 9%, 13%, 35%, 38% at low temperature (25°C). It was found that the metamorphosed juveniles with averaged body length of 5.33±0.04 mm on the larvae fed T. chui at day 19, which significantly higher than the corresponding 19-day-age length values of larvae fed T. weissflogii (4.76±0.09 mm), I. galbana (3.68±0.29 mm) and C. muelleri (3.28 ± 0.15 mm) at 29° C. At 25° C, the metamorphosed juveniles appeared on day 19 with averaged body length of 5.30±0.14 mm for those fed T. chui, which were significantly higher than those larvae on day 19 fed T. weissflogii (4.90 ± 0.02 mm), I. galbana (4.02 ± 0.01 mm) and Chaetoceros muelleri (3.28 ± 0.15 mm) respectively. At 29 °C and at day 19, the average length of pereiopod were 1.15±0.21 mm, 0.95±0.09 mm, 0.68±0.03 mm and 0.62±0.02 mm for T. chui, T. weissflogii, I. galbana and C. muelleri respectively. At 25°C and at day 19, in the treatment of Tetraselmis chui, the average length of pereiopods were 1.28 ± 0.12 mm while it was 0.45 ± 0.05 mm, 0.68 ± 0.08 mm and 0.95 ± 0.12 mm for C. muelleri, I. galbana and T. weissflogii respectively. At 29 °C and at day 19, in the treatment of Tetraselmis chui, the average length of pleopod were 0.84 ± 0.06 mm while it was 0.72 ± 0.04 mm, 0.22 ± 0.09 mm and 0.13 ± 0.02 mm for T. weissflogii, I. galbana and C. muelleri respectively. At 25 °C and at day 19, the results were 0.82 ± 0.07 mm, 0.72 ± 0.09 mm, 0.29 ± 0.01 mm and 0.31 ± 0.01 mm for T. chui, T. weissflogii, I. galbana and C. muelleri. In conclusion, both C. muelleri and I. galbana were recommended live diets for planktonic larvae of C. multidentata when culture condition was well control for: temperature 25-29℃, larvae density 30 individuals/L, algae density 2 x 105 cells/ ml, salinity 20‰. Keywords: Algae, Caridina multidentata, development, growth, larvae culture, Ornamental shrimp,