Characterization and Immobilization of Recombinant L-Ribose Isomerase from Actinotalea fermentans ATCC 43279 and Altering Its Thermostability by Protein Engineering
碩士 === 國立臺灣海洋大學 === 食品科學系 === 105 === A putative L-ribose isomerase gene of A. fermentans ATCC 43279 was chemically synthesized, subcloned into pET-21b vector, and then overexpressed in Escherichia coli. The purified recombinant L-ribose isomerase (Af-RI) demonstrated its optimal activity at 45ºC an...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2017
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| Online Access: | http://ndltd.ncl.edu.tw/handle/kpf779 |