Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment

• Main Authors: Tsai, Shiang-Yu, 蔡翔宇
• Other Authors: Hsu, Pang-Hung
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/8b26y5

碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 105 === Phosphorus plays decisive roles in living organism such as being the building block of cell membrane, and the genetic information storage. In order to investigate how phosphorus availability affects cellular functions at the protein level in diatoms, a modified stable isotope labeling by amino acids in cell culture (SILAC) method coupled with mass spectrometry based quantitative proteomics approach was performed in this study. Phaeodactylum tricornutum serves as a good model organism for understanding the environmental impact to cellular responses in diatoms because of the available comprehensive genomics and proteomics information. The stable isotope of nitrogen, 15N, was introduced in the culture medium as the nitrogen source for protein labeling. The protein profiles of Phaeodactylum tricornutum were evaluated by LC-MS/MS analysis.