Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment
碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 105 === Phosphorus plays decisive roles in living organism such as being the building block of cell membrane, and the genetic information storage. In order to investigate how phosphorus availability affects cellular functions at the protein level in diatoms, a mod...
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ndltd-TW-105NTOU56130142019-05-16T00:00:46Z http://ndltd.ncl.edu.tw/handle/8b26y5 Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment 三角褐指藻於缺磷環境下之蛋白質體定量 Tsai, Shiang-Yu 蔡翔宇 碩士 國立臺灣海洋大學 生命科學暨生物科技學系 105 Phosphorus plays decisive roles in living organism such as being the building block of cell membrane, and the genetic information storage. In order to investigate how phosphorus availability affects cellular functions at the protein level in diatoms, a modified stable isotope labeling by amino acids in cell culture (SILAC) method coupled with mass spectrometry based quantitative proteomics approach was performed in this study. Phaeodactylum tricornutum serves as a good model organism for understanding the environmental impact to cellular responses in diatoms because of the available comprehensive genomics and proteomics information. The stable isotope of nitrogen, 15N, was introduced in the culture medium as the nitrogen source for protein labeling. The protein profiles of Phaeodactylum tricornutum were evaluated by LC-MS/MS analysis. Hsu, Pang-Hung 許邦弘 2017 學位論文 ; thesis 42 zh-TW |
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碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 105 === Phosphorus plays decisive roles in living organism such as being the building block of cell membrane, and the genetic information storage. In order to investigate how phosphorus availability affects cellular functions at the protein level in diatoms, a modified stable isotope labeling by amino acids in cell culture (SILAC) method coupled with mass spectrometry based quantitative proteomics approach was performed in this study. Phaeodactylum tricornutum serves as a good model organism for understanding the environmental impact to cellular responses in diatoms because of the available comprehensive genomics and proteomics information. The stable isotope of nitrogen, 15N, was introduced in the culture medium as the nitrogen source for protein labeling. The protein profiles of Phaeodactylum tricornutum were evaluated by LC-MS/MS analysis.
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author2 |
Hsu, Pang-Hung |
author_facet |
Hsu, Pang-Hung Tsai, Shiang-Yu 蔡翔宇 |
author |
Tsai, Shiang-Yu 蔡翔宇 |
spellingShingle |
Tsai, Shiang-Yu 蔡翔宇 Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment |
author_sort |
Tsai, Shiang-Yu |
title |
Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment |
title_short |
Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment |
title_full |
Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment |
title_fullStr |
Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment |
title_full_unstemmed |
Quantitative Proteomic of Phaeodactylum tricornutum in Phosphate Limited Environment |
title_sort |
quantitative proteomic of phaeodactylum tricornutum in phosphate limited environment |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/8b26y5 |
work_keys_str_mv |
AT tsaishiangyu quantitativeproteomicofphaeodactylumtricornutuminphosphatelimitedenvironment AT càixiángyǔ quantitativeproteomicofphaeodactylumtricornutuminphosphatelimitedenvironment AT tsaishiangyu sānjiǎohèzhǐzǎoyúquēlínhuánjìngxiàzhīdànbáizhìtǐdìngliàng AT càixiángyǔ sānjiǎohèzhǐzǎoyúquēlínhuánjìngxiàzhīdànbáizhìtǐdìngliàng |
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1719159359601115136 |