The improvement effect of yogurt containing mainly Bifidobacterium longum on gut microbiota in rats

• Main Authors: Sheng-Fang Huang, 黃聖方
• Other Authors: 潘子明
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/28y8s8

碩士 === 國立臺灣大學 === 生化科技學系 === 105 === Gut microbiota plays an important role in our body, it have several functions, such as immune regulation, blood lipid and blood glucose regulation, nutrients production and energy required for intestinal cells. And it provides a barrier to against pathogen invasion. Gut microbiota dysbiosis may lead diseases, as probiotics and fermented milk could rebalance gut microbiota in previous studies. In this study, using fermented yogurt containing Bifidobacterium longum as sample (NY yogurt) to understand the intestinal beneficial effect of different doses. Sprague-Dawley (SD) rats were orally administrated with 2.2 g/kg BW, 4.4 g/kg BW and 8.8 g/kg BW yogurt powder per day (i.e. 9.3 g/kg BW, 18.6 g/kg BW and 37.2 g/kg BW yogurt; 3.40 × 104 cfu/kg BW, 6.80 × 104 cfu/kg BW and 1.36 × 105 cfu/kg BW B. longum), respectively. This result shows that feeding B. longum contenting yogurt can increase fecal Bifidobacterium spp. and Lactobacillus spp. contents, and decrease the numbers of Enterobacteriaceae and Clostridium perfringens in cecum and feces. It displays that this yogurt has improvement effect on gut microbiota. In addition, feeding NY yogurt can increase intestinal transit rate, reduce fecal pH value, increase fecal moisture, and increase caecum and fecal short-chain fatty acid contents. Thereby, it shows the enhancing effects of intestinal health. Based on the above results, the fermented yogurt containing B. longum can improve fecal condition and intestinal peristalsis by adjusting gut microbiota, and enhancing the short-chain fatty acids to promote intestinal health.