| Summary: | 碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 105 === Peritoneal dialysis is a treatment for patients who suffer from severe chronic kidney disease. To prevent any infection during the treatment, it is important to monitor the population of white blood cell in peritoneal dialysis. At present, fluorescence-based flow cytometry and the automated hemocytometer are two prevailing methods to quantify the population of white blood cell. However, these techniques usually exist several limitations, such as (1) cannot deal with low white blood cell level (<300 cells/μL), (2) laborious assay preparation and manipulation steps. To address the above problems, we develop a microfluidic device with hydrodynamic trap arrays to capture white blood cells. The microfluidic microtrap array with multiple dimensions can trap general white blood cells and specific white blood cell subpopulation conjugated with 30μm polystyrene beads. This microfluidic platform enables simultaneously cell trapping, selection and can perform simple and real-time cell counting without complicated sample processing steps and equipment. To make the system more user-friendly and be suitable for point-of-care (POC) settings, we plan to make this microfluidic device compatible to existing smartphones and APP, which can perform image processing, analysis and data transmission. We believe this microfluidic platform for surveillance of white blood cell level will hold significant promise to provide the detailed infection status of patient for doctors to perform timely treatment.
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