An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome
碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 105 === In this thesis, we have developed an automatic microfluidic DNA microarray system for single nucleotide polymorphism (SNP) screening for Long QT syndrome (LQTS). LQTS is a genetic heart disease caused by SNPs and genetic screening is the most effective diagn...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | en_US |
Published: |
2016
|
Online Access: | http://ndltd.ncl.edu.tw/handle/40779737845238885104 |
id |
ndltd-TW-105NTU05114005 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-105NTU051140052017-03-25T04:37:13Z http://ndltd.ncl.edu.tw/handle/40779737845238885104 An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome 開發自動化微流道DNA微陣列晶片應用於長Q-T症候群之單核苷酸多型性檢測 Yu-Shin Chang 張郁欣 碩士 國立臺灣大學 生醫電子與資訊學研究所 105 In this thesis, we have developed an automatic microfluidic DNA microarray system for single nucleotide polymorphism (SNP) screening for Long QT syndrome (LQTS). LQTS is a genetic heart disease caused by SNPs and genetic screening is the most effective diagnosis method. DNA microarray is one of the most powerful methods which can screen thousands of genes on a single chip. However, conventional DNA microarray is time-consuming (at least 16 hours) since the target and probe DNA hybridization is based on the passive diffusion. To address this problem, we integrated a microfluidic system with DNA microarray to reduce the hybridization time from 16 to 2 hours by active mixing and minimizing the manual operation process. Our study first optimized the hybridization conditions, including hybridization temperature and sample flow rate. Then, to differentiate the SNPs between wild type and mutation probes, we placed an additional mismatch on both types of probes. The results showed that the SNPs could be effectively detected (or differentiated). Compared to current DNA microarray methods, automatic microfluidic DNA microarray can minimize the manual manipulation. Besides, by reducing the assay reagent volume and using the active mixing in the hybridization process, the total assay time can be reduced to 3 hours, which is eight times shorter than conventional DNA microarray. With the higher selectivity of SNPs, short hybridization time, and automatic procedure, we believe that our device has the potential to achieve high-throughput, rapid and sensitive gene screening test. Nien-Tsu Huang 黃念祖 2016 學位論文 ; thesis 72 en_US |
collection |
NDLTD |
language |
en_US |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 105 === In this thesis, we have developed an automatic microfluidic DNA microarray system for single nucleotide polymorphism (SNP) screening for Long QT syndrome (LQTS). LQTS is a genetic heart disease caused by SNPs and genetic screening is the most effective diagnosis method. DNA microarray is one of the most powerful methods which can screen thousands of genes on a single chip. However, conventional DNA microarray is time-consuming (at least 16 hours) since the target and probe DNA hybridization is based on the passive diffusion. To address this problem, we integrated a microfluidic system with DNA microarray to reduce the hybridization time from 16 to 2 hours by active mixing and minimizing the manual operation process. Our study first optimized the hybridization conditions, including hybridization temperature and sample flow rate. Then, to differentiate the SNPs between wild type and mutation probes, we placed an additional mismatch on both types of probes. The results showed that the SNPs could be effectively detected (or differentiated). Compared to current DNA microarray methods, automatic microfluidic DNA microarray can minimize the manual manipulation. Besides, by reducing the assay reagent volume and using the active mixing in the hybridization process, the total assay time can be reduced to 3 hours, which is eight times shorter than conventional DNA microarray. With the higher selectivity of SNPs, short hybridization time, and automatic procedure, we believe that our device has the potential to achieve high-throughput, rapid and sensitive gene screening test.
|
author2 |
Nien-Tsu Huang |
author_facet |
Nien-Tsu Huang Yu-Shin Chang 張郁欣 |
author |
Yu-Shin Chang 張郁欣 |
spellingShingle |
Yu-Shin Chang 張郁欣 An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome |
author_sort |
Yu-Shin Chang |
title |
An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome |
title_short |
An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome |
title_full |
An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome |
title_fullStr |
An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome |
title_full_unstemmed |
An Automatic Microfluidic DNA Microarray Platform for Single Nucleotide Polymorphism Screening of Long QT Syndrome |
title_sort |
automatic microfluidic dna microarray platform for single nucleotide polymorphism screening of long qt syndrome |
publishDate |
2016 |
url |
http://ndltd.ncl.edu.tw/handle/40779737845238885104 |
work_keys_str_mv |
AT yushinchang anautomaticmicrofluidicdnamicroarrayplatformforsinglenucleotidepolymorphismscreeningoflongqtsyndrome AT zhāngyùxīn anautomaticmicrofluidicdnamicroarrayplatformforsinglenucleotidepolymorphismscreeningoflongqtsyndrome AT yushinchang kāifāzìdònghuàwēiliúdàodnawēizhènlièjīngpiànyīngyòngyúzhǎngqtzhènghòuqúnzhīdānhégānsuānduōxíngxìngjiǎncè AT zhāngyùxīn kāifāzìdònghuàwēiliúdàodnawēizhènlièjīngpiànyīngyòngyúzhǎngqtzhènghòuqúnzhīdānhégānsuānduōxíngxìngjiǎncè AT yushinchang automaticmicrofluidicdnamicroarrayplatformforsinglenucleotidepolymorphismscreeningoflongqtsyndrome AT zhāngyùxīn automaticmicrofluidicdnamicroarrayplatformforsinglenucleotidepolymorphismscreeningoflongqtsyndrome |
_version_ |
1718434662259359744 |