Glucose Monitoring System with Hematocrit Error Compensation Based on Voltage-Induced Hemolysis

• Main Authors: Chih-Wei Weng, 翁志偉
• Other Authors: 鄭宗記
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/z7wk5r

博士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 105 === Besides a crucial parameter for surgery and clinical diagnosis, hematocrit tends to affect the analytical result of point-of-care analytical devices. Therefore, an accurate and quick method for measuring hematocrit was developed on the basis of screen-printed carbon electrodes (SPCE). An impulse of 3.0V DC voltage was imposed on ferricyanide-coated SPCE to induce hemolysis, the released hemoglobin reduced the ferricyanide and generated a higher oxidation current for estimating hematocrit. Hematocrit ranging from 10 to 70% can be determined in 5s by linear sweep voltammetry (r2= 0.9907) or 0.8s by 3V of potential step voltammetry (r2 = 0.9833). Furthermore, accurate and quick disposable sensor for both glucose and hematocrit (Hct) was also developed. The aim of this study was to develop a method for glucose calculation with improved accuracy using the Hct compensation method that minimizes the effects of Hct on glucose measurements. Typically, simple chronoamperometry with one potential step is used to measure glucose concentrations. The mean biases of glucose measurements were linearly correlated with Hcts from 9% to 70% and the deviations ranged from 40% to -50%. The result indicated that simple chronoamperometry method for glucose measurements were highly Hct dependent (R2 = 0.992). The present study showed that an additional potential step of 3.2V not only can be used for Hct detection but also applied for interference compensation of glucose measurement. After compensation, the biases of glucose measurements of proposed method were Hct independent (R2 = 0.0705) and is consequently capable of operating acceptably in a wide Hct range of 10% ~ 70% with biases in ± 10% which has improved the accuracy of glucose measurements as compared with the existing chronoamperometry method.